Variation in the metabolites and α-glucosidase inhibitory activity of Cosmos caudatus at different growth stages.

Asteraceae / chemistry Glycoside Hydrolase Inhibitors / chemistry Plant Extracts / chemistry Plant Leaves / chemistry Tandem Mass Spectrometry alpha-Glucosidases / chemistry
Cosmos caudatus Harvesting age NMR-based metabolomics Quercetin derivatives Total phenolic content α-Glucosidase inhibition

Journal

BMC complementary and alternative medicine
ISSN: 1472-6882
Titre abrégé: BMC Complement Altern Med
Pays: England
ID NLM: 101088661

Informations de publication

Date de publication:
05 Sep 2019
Historique:
received: 11 09 2018
accepted: 27 08 2019
entrez: 7 9 2019
pubmed: 7 9 2019
medline: 31 12 2019
Statut: epublish

Résumé

Cosmos caudatus is an annual plant known for its medicinal value in treating several health conditions, such as high blood pressure, arthritis, and diabetes mellitus. The α-glucosidase inhibitory activity and total phenolic content of the leaf aqueous ethanolic extracts of the plant at different growth stages (6, 8. 10, 12 and 14 weeks) were determined in an effort to ascertain the best time to harvest the plant for maximum medicinal quality with respect to its glucose-lowering effects. The aqueous ethanolic leaf extracts of C. caudatus were characterized by NMR and LC-MS/MS. The total phenolic content and α-glucosidase inhibitory activity were evaluated by the Folin-Ciocalteu method and α-glucosidase inhibitory assay, respectively. The statistical significance of the results was evaluated using one-way ANOVA with Duncan's post hoc test, and correlation among the different activities was performed by Pearson's correlation test. NMR spectroscopy along with multivariate data analysis was used to identify the metabolites correlated with total phenolic content and α-glucosidase inhibitory activity of the C. caudatus leaf extracts. It was found that the α-glucosidase inhibitory activity and total phenolic content of the optimized ethanol:water (80:20) leaf extract of the plant increased significantly as the plant matured, reaching a maximum at the 10th week. The IC For high phytomedicinal quality, the 10th week is recommended as the best time to harvest C. caudatus leaves with respect to its glucose lowering potential.

Sections du résumé

BACKGROUND BACKGROUND
Cosmos caudatus is an annual plant known for its medicinal value in treating several health conditions, such as high blood pressure, arthritis, and diabetes mellitus. The α-glucosidase inhibitory activity and total phenolic content of the leaf aqueous ethanolic extracts of the plant at different growth stages (6, 8. 10, 12 and 14 weeks) were determined in an effort to ascertain the best time to harvest the plant for maximum medicinal quality with respect to its glucose-lowering effects.
METHODS METHODS
The aqueous ethanolic leaf extracts of C. caudatus were characterized by NMR and LC-MS/MS. The total phenolic content and α-glucosidase inhibitory activity were evaluated by the Folin-Ciocalteu method and α-glucosidase inhibitory assay, respectively. The statistical significance of the results was evaluated using one-way ANOVA with Duncan's post hoc test, and correlation among the different activities was performed by Pearson's correlation test. NMR spectroscopy along with multivariate data analysis was used to identify the metabolites correlated with total phenolic content and α-glucosidase inhibitory activity of the C. caudatus leaf extracts.
RESULTS RESULTS
It was found that the α-glucosidase inhibitory activity and total phenolic content of the optimized ethanol:water (80:20) leaf extract of the plant increased significantly as the plant matured, reaching a maximum at the 10th week. The IC
CONCLUSION CONCLUSIONS
For high phytomedicinal quality, the 10th week is recommended as the best time to harvest C. caudatus leaves with respect to its glucose lowering potential.

Identifiants

DOI: 10.1186/s12906-019-2655-9 PMID: 31488132 PMC: PMC6727405
pubmed: 31488132
doi: 10.1186/s12906-019-2655-9
pii: 10.1186/s12906-019-2655-9
pmc: PMC6727405
doi:

Substances chimiques

Glycoside Hydrolase Inhibitors 0
Plant Extracts 0
alpha-Glucosidases EC 3.2.1.20

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

245

Subventions

Organisme : This project is funded by Ministry of Science Malaysia, Technology and Innovation (MOSTI)
ID : grant no: 637007

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Auteurs

Wan Ahmad Wan-Nadilah (WA)

Faculty of Bioresources and Food Industry, Universiti Sultan Zainal Abidin, Besut, Terengganu, Malaysia.
Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.

Muhammad Tayyab Akhtar (MT)

Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.
Institute of Industrial Biotechnology, Government College University, Lahore, Pakistan.

Khozirah Shaari (K)

Laboratory of Natural Products, Institute of Bioscience, Universiti Putra Malaysia, Serdang, Selangor, Malaysia. khozirah@yahoo.com.my.
ORCID: 0000-0002-7054-0649

Alfi Khatib (A)

Kuliyyah of Pharmacy, International Islamic University Malaysia, Kuantan, Pahang, Malaysia.

Azizah Abdul Hamid (AA)

Faculty of Food Science and Technology, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.

Muhajir Hamid (M)

Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Asteraceae › Variation in the metabolites and α-glucosidase...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Effets physiologiques des médicaments › Hypoglycémiants › Inhibiteurs des glycoside hydrolases › Variation in the metabolites and α-glucosidase...
questionsmedicales.fr › Préparations pharmaceutiques › Extraits de plantes › Variation in the metabolites and α-glucosidase...
questionsmedicales.fr › Structures de plante › Parties aériennes de plante › Feuilles de plante › Variation in the metabolites and α-glucosidase...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Spectrométrie de masse en tandem › Variation in the metabolites and α-glucosidase...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Glycosidases › Glucosidases › alpha-Glucosidase › Variation in the metabolites and α-glucosidase...