Plasma concentrations of steroid precursors, steroids, neuroactive steroids, and neurosteroids in healthy neonatal foals from birth to 7 days of age.
Animals
Animals, Newborn
/ blood
Dehydroepiandrosterone
/ blood
Dehydroepiandrosterone Sulfate
/ blood
Desoxycorticosterone
/ blood
Female
Horses
/ blood
Hypothalamo-Hypophyseal System
/ physiology
Male
Pituitary-Adrenal System
/ physiology
Pregnenolone
/ blood
Progesterone
/ blood
Prospective Studies
brain
equine
maladjustment
progesterone
progestins
Journal
Journal of veterinary internal medicine
ISSN: 1939-1676
Titre abrégé: J Vet Intern Med
Pays: United States
ID NLM: 8708660
Informations de publication
Date de publication:
Sep 2019
Sep 2019
Historique:
received:
28
05
2019
accepted:
26
08
2019
pubmed:
7
9
2019
medline:
6
2
2020
entrez:
7
9
2019
Statut:
ppublish
Résumé
Transient hypothalamic-pituitary-adrenal axis dysfunction occurs in critically ill foals with sepsis and neonatal maladjustment syndrome (NMS). Cortisol is the most commonly measured steroid. However, a complex interaction of various steroid compounds might play a role in pathophysiology of this disorder. To identify steroid compounds present at high concentrations at birth that rapidly and steadily decrease within the first 7 days of life in healthy foals and that might be supportive diagnosis of NMS and other neonatal disorders. Ten healthy neonatal Quarter Horse foals (5 females and 5 males). Prospective study. Blood was collected in heparinized tubes within 30 minutes after birth, and at 12, 24, 48, 72, 96, 120, 144, and 168 hours of age. Plasma was separated and a panel of steroid compounds was analyzed using liquid chromatography-mass spectrometry. A nonlinear regression model was used to determine decay concentrations over time. Confidence intervals (CIs) were calculated and significance was set a P ≤ .05. Five compounds were identified: pregnenolone, progesterone, deoxycorticosterone, dehydroepiandrosterone, and dehydroepiandrosterone sulfate. Pregnenolone and progesterone concentrations rapidly decreased by 24 hours of age and remained low throughout the first 7 days of life. Their half-life (95% CI) was short at 3.7 (3.4, 4.0) and 4.5 (2.8, 6.1) hours, respectively. No statistical differences in the concentrations of these compounds were found between males and females. Progesterone might be a useful marker for identifying continuous endogenous production of neuroactive steroids in foals with suspected NMS and other neonatal diseases.
Sections du résumé
BACKGROUND
BACKGROUND
Transient hypothalamic-pituitary-adrenal axis dysfunction occurs in critically ill foals with sepsis and neonatal maladjustment syndrome (NMS). Cortisol is the most commonly measured steroid. However, a complex interaction of various steroid compounds might play a role in pathophysiology of this disorder.
OBJECTIVE
OBJECTIVE
To identify steroid compounds present at high concentrations at birth that rapidly and steadily decrease within the first 7 days of life in healthy foals and that might be supportive diagnosis of NMS and other neonatal disorders.
ANIMALS
METHODS
Ten healthy neonatal Quarter Horse foals (5 females and 5 males).
METHODS
METHODS
Prospective study. Blood was collected in heparinized tubes within 30 minutes after birth, and at 12, 24, 48, 72, 96, 120, 144, and 168 hours of age. Plasma was separated and a panel of steroid compounds was analyzed using liquid chromatography-mass spectrometry. A nonlinear regression model was used to determine decay concentrations over time. Confidence intervals (CIs) were calculated and significance was set a P ≤ .05.
RESULTS
RESULTS
Five compounds were identified: pregnenolone, progesterone, deoxycorticosterone, dehydroepiandrosterone, and dehydroepiandrosterone sulfate. Pregnenolone and progesterone concentrations rapidly decreased by 24 hours of age and remained low throughout the first 7 days of life. Their half-life (95% CI) was short at 3.7 (3.4, 4.0) and 4.5 (2.8, 6.1) hours, respectively. No statistical differences in the concentrations of these compounds were found between males and females.
CONCLUSIONS AND CLINICAL RELEVANCE
CONCLUSIONS
Progesterone might be a useful marker for identifying continuous endogenous production of neuroactive steroids in foals with suspected NMS and other neonatal diseases.
Identifiants
pubmed: 31489708
doi: 10.1111/jvim.15618
pmc: PMC6766486
doi:
Substances chimiques
Desoxycorticosterone
40GP35YQ49
Dehydroepiandrosterone
459AG36T1B
Progesterone
4G7DS2Q64Y
Dehydroepiandrosterone Sulfate
57B09Q7FJR
Pregnenolone
73R90F7MQ8
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2286-2293Informations de copyright
© 2019 The Authors. Journal of Veterinary Internal Medicine published by Wiley Periodicals, Inc. on behalf of the American College of Veterinary Internal Medicine.
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