EPS bound flavins driven mediated electron transfer in thermophilic Geobacillus sp.


Journal

Microbiological research
ISSN: 1618-0623
Titre abrégé: Microbiol Res
Pays: Germany
ID NLM: 9437794

Informations de publication

Date de publication:
Dec 2019
Historique:
received: 28 05 2019
revised: 09 08 2019
accepted: 23 08 2019
pubmed: 7 9 2019
medline: 9 11 2019
entrez: 7 9 2019
Statut: ppublish

Résumé

Through extracellular electron transfer (EET), bacteria are capable of transforming different insoluble materials of geochemical interest into energy-rich molecules for their growth. For this process, bacteria have been depending directly or indirectly on molecules synthesized within the cells or by various synthetics as mediators. Herein, we studied the in-situ change in electrochemistry and supporting components for EET in the extracellular polysaccharide (EPS) producing biofilm of thermophilic Geobacillus sp. The CV and DPV resultsrevealed that the intact biofilm of bacteria was not able to generate any potential at 25 °C /- ≤50 °C. However, at 55 °C (optimal condition), the potential occurred drastically after the EPS production by bacteria. HPLC and MALDI-TOF results revealed that the presence of Flavins, which can able adsorbed to the electrodes from the cell surface. Moreover, the temperature-dependent EPS production and originally conceived ability of flavins to act as electron shuttles suggest that not much complexity in bacteria with minerals. Additionally, the electrochemical potential was severely affected upon removal of EPS/flavin moiety from the intact biofilm, revealed the necessity of EPS bound flavins in transferring the electrons across its thick cell walls. This paradigm shift to electrogenic nature of Geobacillus sp. biofilm will become evident in the adaptation of other microbes during mineral respiration in extreme environments.

Identifiants

pubmed: 31491671
pii: S0944-5013(19)30577-4
doi: 10.1016/j.micres.2019.126324
pii:
doi:

Substances chimiques

Flavins 0
Polysaccharides, Bacterial 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

126324

Informations de copyright

Copyright © 2019 Elsevier GmbH. All rights reserved.

Auteurs

Dummi Mahadevan Gurumurthy (DM)

Department of Biotechnology, G.M. Institute of Technology, Davangere, 577006, India. Electronic address: drgurumurthydm@gmit.ac.in.

Ram Naresh Bharagava (RN)

Department of Microbiology (DM), School for Environmental Sciences (SES), Babasaheb BhimraoAmbedkar University (A Central University), VidyaVihar, Raebareli Road, Lucknow 226 025, Uttar Pradesh, India.

Ashok Kumar (A)

Department of Biotechnology and Bioinformatics, Jaypee University of Information Technology, Waknaghat, Solan, Himachal Pradesh, 173234, India.

Bhaskar Singh (B)

Department of Environmental Sciences, Central University of Jharkhand, Ranchi, 835205, Jharkhand, India.

Muhammad Ashfaq (M)

Department of Chemistry, University of Gujrat, Gujrat, 50700, Pakistan.

Ganesh Dattatraya Saratale (GD)

Department of Food Science and Biotechnology, Dongguk University-Seoul, Ilsandong-gu, Goyang-si, Gyeonggi-do, 10326, Republic of Korea.

Sikandar I Mulla (SI)

Department of Biochemistry, School of Applied Sciences, REVA University, Bangalore, 560 064, Karnataka State, India. Electronic address: sikandar.mulla@gmail.com.

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Classifications MeSH