EPS bound flavins driven mediated electron transfer in thermophilic Geobacillus sp.
Extracellular electron transfer
Extracellular polysaccharide
Riboflavin
Thermophiles
Journal
Microbiological research
ISSN: 1618-0623
Titre abrégé: Microbiol Res
Pays: Germany
ID NLM: 9437794
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
received:
28
05
2019
revised:
09
08
2019
accepted:
23
08
2019
pubmed:
7
9
2019
medline:
9
11
2019
entrez:
7
9
2019
Statut:
ppublish
Résumé
Through extracellular electron transfer (EET), bacteria are capable of transforming different insoluble materials of geochemical interest into energy-rich molecules for their growth. For this process, bacteria have been depending directly or indirectly on molecules synthesized within the cells or by various synthetics as mediators. Herein, we studied the in-situ change in electrochemistry and supporting components for EET in the extracellular polysaccharide (EPS) producing biofilm of thermophilic Geobacillus sp. The CV and DPV resultsrevealed that the intact biofilm of bacteria was not able to generate any potential at 25 °C /- ≤50 °C. However, at 55 °C (optimal condition), the potential occurred drastically after the EPS production by bacteria. HPLC and MALDI-TOF results revealed that the presence of Flavins, which can able adsorbed to the electrodes from the cell surface. Moreover, the temperature-dependent EPS production and originally conceived ability of flavins to act as electron shuttles suggest that not much complexity in bacteria with minerals. Additionally, the electrochemical potential was severely affected upon removal of EPS/flavin moiety from the intact biofilm, revealed the necessity of EPS bound flavins in transferring the electrons across its thick cell walls. This paradigm shift to electrogenic nature of Geobacillus sp. biofilm will become evident in the adaptation of other microbes during mineral respiration in extreme environments.
Identifiants
pubmed: 31491671
pii: S0944-5013(19)30577-4
doi: 10.1016/j.micres.2019.126324
pii:
doi:
Substances chimiques
Flavins
0
Polysaccharides, Bacterial
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
126324Informations de copyright
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