Phlda3 regulates beta cell survival during stress.
Animals
Antioxidants
/ pharmacology
Cell Death
/ drug effects
Cell Line
Cell Survival
/ drug effects
Cytokines
/ pharmacology
Cytoprotection
/ drug effects
Endoplasmic Reticulum Chaperone BiP
Endoplasmic Reticulum Stress
/ drug effects
Female
Humans
Insulin-Secreting Cells
/ cytology
Mice, Inbred C57BL
Mice, Inbred NOD
Models, Biological
NF-kappa B
/ metabolism
Nuclear Proteins
/ genetics
Oxidative Stress
/ drug effects
Palmitic Acid
/ pharmacology
RNA, Messenger
/ genetics
Stress, Physiological
/ drug effects
Thapsigargin
/ pharmacology
Unfolded Protein Response
/ drug effects
Up-Regulation
/ drug effects
X-Box Binding Protein 1
/ metabolism
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
06 09 2019
06 09 2019
Historique:
received:
21
02
2019
accepted:
19
08
2019
entrez:
8
9
2019
pubmed:
8
9
2019
medline:
23
10
2020
Statut:
epublish
Résumé
The loss of functional beta cell mass characterises all forms of diabetes. Beta cells are highly susceptible to stress, including cytokine, endoplasmic reticulum (ER) and oxidative stress. This study examined the role of pleckstrin homology-like, domain family A, member 3 (Phlda3) in beta cell survival under stress conditions and the regulatory basis. We found that the mRNA levels of Phlda3 were markedly upregulated in vivo in the islets of diabetic humans and mice. In vitro, exposure of MIN6 cells or islets to cytokines, palmitate, thapsigargin or ribose upregulated Phlda3 mRNA and protein levels, concurrent with the induction of ER stress (Ddit3 and Trb3) and antioxidant (Hmox1) genes. Furthermore, H
Identifiants
pubmed: 31492921
doi: 10.1038/s41598-019-49289-5
pii: 10.1038/s41598-019-49289-5
pmc: PMC6731300
doi:
Substances chimiques
Antioxidants
0
Cytokines
0
Endoplasmic Reticulum Chaperone BiP
0
HSPA5 protein, human
0
Hspa5 protein, mouse
0
NF-kappa B
0
Nuclear Proteins
0
RNA, Messenger
0
TSSC3 protein
0
X-Box Binding Protein 1
0
Palmitic Acid
2V16EO95H1
Thapsigargin
67526-95-8
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
12827Références
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