Efficient data acquisition with three-channel centerpieces in sedimentation velocity.
3D printing
Analytical ultracentrifugation
Laboratory automation
Sedimentation velocity
Journal
Analytical biochemistry
ISSN: 1096-0309
Titre abrégé: Anal Biochem
Pays: United States
ID NLM: 0370535
Informations de publication
Date de publication:
01 12 2019
01 12 2019
Historique:
received:
25
07
2019
revised:
29
08
2019
accepted:
31
08
2019
pubmed:
8
9
2019
medline:
11
8
2020
entrez:
8
9
2019
Statut:
ppublish
Résumé
Three-channel 3D printed centerpieces with two sample sectors next to a joint solvent reference sector were recently described as a strategy to double the throughput of sedimentation velocity analytical ultracentrifugation experiments [Anal. Chem. 91 (2019) 5866-5873]. They are compatible with Rayleigh interference optical detection in commercial analytical ultracentrifuges, but require the rotor angles of data acquisition to be repeatedly adjusted during the experiment to record data from the two sample sectors. Here we present an approach to automate this data acquisition mode through the use of a secondary, general-purpose automation software, and an accompanying data pre-processing software for scan sorting.
Identifiants
pubmed: 31493371
pii: S0003-2697(19)30732-8
doi: 10.1016/j.ab.2019.113414
pmc: PMC6768728
mid: NIHMS1539915
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Intramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
113414Subventions
Organisme : Intramural NIH HHS
ID : ZIA EB000051-12
Pays : United States
Informations de copyright
Published by Elsevier Inc.
Références
Biochem Soc Trans. 1998 Nov;26(4):753-8
pubmed: 10047821
Biophys J. 1999 Apr;76(4):2288-96
pubmed: 10096923
Anal Biochem. 2000 Oct 1;285(1):135-42
pubmed: 10998273
J Biol Chem. 2003 Aug 15;278(33):30936-44
pubmed: 12771151
Arch Biochem Biophys. 1962 Oct;99:157-74
pubmed: 13952541
Anal Biochem. 2007 Feb 1;361(1):24-30
pubmed: 17181992
AAPS J. 2008 Sep;10(3):481-93
pubmed: 18814037
Eur Biophys J. 2009 Oct;38(8):1079-99
pubmed: 19644686
Anal Biochem. 2010 Jun 15;401(2):280-7
pubmed: 20206114
Nat Commun. 2011 Jun 07;2:335
pubmed: 21654635
J Microsc. 2011 Nov;244(2):181-6
pubmed: 21810094
Small. 2012 Jul 23;8(14):2277-86
pubmed: 22517616
Curr Protoc Protein Sci. 2013 Feb;Chapter 20:Unit20.12
pubmed: 23377850
Anal Biochem. 2013 Jun 1;437(1):104-8
pubmed: 23458356
Biophys Rev. 2013 Jun 1;5(2):159-171
pubmed: 23682298
J Pharm Sci. 2013 Sep;102(9):3089-99
pubmed: 23794522
Anal Chem. 2014 Mar 18;86(6):3181-7
pubmed: 24552356
PLoS One. 2015 Mar 24;10(3):e0120820
pubmed: 25803582
PLoS One. 2015 May 21;10(5):e0126420
pubmed: 25997164
Langmuir. 2016 Apr 26;32(16):3926-36
pubmed: 27031248
AAPS J. 2016 Jul;18(4):849-60
pubmed: 27184576
PLoS One. 2016 Aug 15;11(8):e0155201
pubmed: 27525659
Anal Biochem. 2017 Aug 15;531:16-19
pubmed: 28529050
Nat Protoc. 2017 Sep;12(9):1777-1791
pubmed: 28771239
Biophys Rev. 2018 Apr;10(2):229-233
pubmed: 29188538
J Pharmacokinet Pharmacodyn. 2018 Apr;45(2):309-327
pubmed: 29423863
Eur Biophys J. 2018 Oct;47(7):697-707
pubmed: 30056489
PLoS One. 2018 Jul 30;13(7):e0201529
pubmed: 30059530
Eur Biophys J. 2018 Oct;47(7):809-813
pubmed: 30159596
Anal Chem. 2018 Sep 18;90(18):10659-10663
pubmed: 30160111
Nat Commun. 2018 Oct 24;9(1):4415
pubmed: 30356043
Anal Chim Acta. 2018 Dec 28;1043:72-80
pubmed: 30392671
Nat Commun. 2018 Nov 21;9(1):4898
pubmed: 30464237
Proc Natl Acad Sci U S A. 2018 Dec 18;115(51):E11914-E11923
pubmed: 30510001
J Am Chem Soc. 2019 Feb 20;141(7):2990-2996
pubmed: 30668114
AAPS J. 2019 Feb 27;21(3):35
pubmed: 30815745
Anal Chem. 2019 May 7;91(9):5866-5873
pubmed: 30933465