The inoculum effect of Escherichia coli expressing mcr-1 or not on colistin activity in a murine model of peritonitis.
Animals
Anti-Bacterial Agents
/ pharmacokinetics
Bacterial Load
/ drug effects
Colistin
/ pharmacokinetics
Disease Models, Animal
Drug Resistance, Bacterial
/ genetics
Escherichia coli
/ drug effects
Escherichia coli Infections
/ drug therapy
Escherichia coli Proteins
/ genetics
Female
Mice
Mice, Inbred ICR
Microbial Sensitivity Tests
Peritonitis
/ drug therapy
Survival Analysis
Antibiotic resistance
Colistin
Escherichia coli
MCR-1
Peritonitis
Pharmacodynamics
Journal
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
ISSN: 1469-0691
Titre abrégé: Clin Microbiol Infect
Pays: England
ID NLM: 9516420
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
received:
14
04
2019
revised:
05
08
2019
accepted:
27
08
2019
pubmed:
9
9
2019
medline:
26
3
2020
entrez:
9
9
2019
Statut:
ppublish
Résumé
Colistin often remains the last resort antibiotic active against carbapenemase-producing Enterobacteriaceae. However, while in vitro inoculum effect has been reported, therapeutic relevance of this phenomenon remains questioned. Ten E. coli strains were used that included the wild-type CFT073 and its transconjugant CFT073-MCR-1 and eight susceptible clinical isolates. Mice with peritonitis were treated for 24 h with colistin sulfate. Bacterial loads were determined in peritoneal fluid (PF) and spleen and colistin-resistant mutants were detected. MICs of colistin against the eight susceptible clinical strains and CFT073 ranged from 0.125 to 0.5 mg/L with an inoculum of 10 Phenotypic colistin resistance in wild-type E. coli due to an increase in inoculum size had a therapeutic impact in mice with peritonitis that was comparable to that observed when the mcr-1 gene was expressed.
Identifiants
pubmed: 31494253
pii: S1198-743X(19)30484-7
doi: 10.1016/j.cmi.2019.08.021
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Escherichia coli Proteins
0
MCR-1 protein, E coli
0
Colistin
Z67X93HJG1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1563.e5-1563.e8Informations de copyright
Copyright © 2019 European Society of Clinical Microbiology and Infectious Diseases. Published by Elsevier Ltd. All rights reserved.