Optimised oligonucleotide substrates to assay XPF-ERCC1 nuclease activity for the discovery of DNA repair inhibitors.

DNA Repair DNA-Binding Proteins / chemistry Dimerization Endonucleases / chemistry Humans Oligonucleotides / chemistry Substrate Specificity Temperature

Journal

Chemical communications (Cambridge, England)
ISSN: 1364-548X
Titre abrégé: Chem Commun (Camb)
Pays: England
ID NLM: 9610838

Informations de publication

Date de publication:
26 Sep 2019
Historique:
pubmed: 10 9 2019
medline: 9 11 2019
entrez: 10 9 2019
Statut: ppublish

Résumé

We report the design and optimisation of novel oligonucleotide substrates for a sensitive fluorescence assay for high-throughput screening and functional studies of the DNA repair enzyme, XPF-ERCC1, with a view to accelerating inhibitor and drug discovery.

Identifiants

DOI: 10.1039/c9cc05476f PMID: 31497827
pubmed: 31497827
doi: 10.1039/c9cc05476f
doi:

Substances chimiques

DNA-Binding Proteins 0
Oligonucleotides 0
Endonucleases EC 3.1.-

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

11671-11674

Subventions

Organisme : Medical Research Council
ID : MR/R009368/1
Pays : United Kingdom

Auteurs

Adam M Thomas (AM)

Department of Chemistry, University of Oxford, Chemistry Research Laboratory, 12 Mansfield Road, Oxford, OX1 3TA, UK. tom.brown@chem.ox.ac.uk and Department of Oncology, MRC-Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DS, UK and The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK.
ORCID: 0000-0002-8780-8795

Sanja Brolih (S)

Department of Oncology, MRC-Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DS, UK.

Joanna F McGouran (JF)

Department of Chemistry, University of Oxford, Chemistry Research Laboratory, 12 Mansfield Road, Oxford, OX1 3TA, UK. tom.brown@chem.ox.ac.uk.
ORCID: 0000-0002-9349-2141

Afaf H El-Sagheer (AH)

Department of Chemistry, University of Oxford, Chemistry Research Laboratory, 12 Mansfield Road, Oxford, OX1 3TA, UK. tom.brown@chem.ox.ac.uk and Chemistry Branch, Department of Science and Mathematics, Faculty of Petroleum and Mining Engineering, Suez University, Suez 43721, Egypt.
ORCID: 0000-0001-8706-1292

Denis Ptchelkine (D)

Department of Oncology, MRC-Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DS, UK.

Morgan Jones (M)

The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK.

Neil Q McDonald (NQ)

The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK and Institute of Structural and Molecular Biology, Department of Biological Sciences, Birkbeck College, Malet Street, London WC1E 7HX, UK.

Peter J McHugh (PJ)

Department of Oncology, MRC-Weatherall Institute of Molecular Medicine, University of Oxford, John Radcliffe Hospital, Oxford, OX3 9DS, UK.
ORCID: 0000-0002-8679-4627

Tom Brown (T)

Department of Chemistry, University of Oxford, Chemistry Research Laboratory, 12 Mansfield Road, Oxford, OX1 3TA, UK. tom.brown@chem.ox.ac.uk.
ORCID: 0000-0002-6538-3036

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Réparation de l'ADN › Optimised oligonucleotide substrates to assay...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines de liaison à l'ADN › Optimised oligonucleotide substrates to assay...
questionsmedicales.fr › Métabolisme › Dimérisation › Optimised oligonucleotide substrates to assay...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Esterases › Endonucleases › Optimised oligonucleotide substrates to assay...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Optimised oligonucleotide substrates to assay...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Nucléotides › Polynucléotides › Oligonucléotides › Optimised oligonucleotide substrates to assay...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Spécificité du substrat › Optimised oligonucleotide substrates to assay...
questionsmedicales.fr › Environnement et santé publique › Environnement › Concepts météorologiques › Atmosphère › Temps (météorologie) › Température › Optimised oligonucleotide substrates to assay...