A novel high maltose-forming α-amylase from Rhizomucor miehei and its application in the food industry.

Bread / analysis Food Industry Hydrogen-Ion Concentration Hydrolysis Maltose / metabolism Pichia / metabolism Rhizomucor / enzymology Starch / metabolism Substrate Specificity Temperature alpha-Amylases / chemistry
Chinese steamed bread High-cell density fermentation Maltose Pichia pastoris Rhizomucor miehei α-Amylases

Journal

Food chemistry
ISSN: 1873-7072
Titre abrégé: Food Chem
Pays: England
ID NLM: 7702639

Informations de publication

Date de publication:
01 Feb 2020
Historique:
received: 10 05 2019
revised: 24 08 2019
accepted: 28 08 2019
pubmed: 10 9 2019
medline: 18 12 2019
entrez: 10 9 2019
Statut: ppublish

Résumé

A novel α-amylase gene (RmAmyA) from Rhizomucor miehei was cloned and expressed in Pichia pastoris. RmAmyA showed 70% amino acid identity with the α-amylase from Rhizomucor pusillus. A high α-amylase activity of 29,794.2 U/mL was found through high cell density fermentation. The molecular mass of RmAmyA was determined to be 49.9 kDa via SDS-PAGE. RmAmyA was optimally active at 75 °C and pH 6.0, and it did not require Ca

Identifiants

DOI: 10.1016/j.foodchem.2019.125447 PMID: 31499289
pubmed: 31499289
pii: S0308-8146(19)31562-6
doi: 10.1016/j.foodchem.2019.125447
pii:
doi:

Substances chimiques

Maltose 69-79-4
Starch 9005-25-8
alpha-Amylases EC 3.2.1.1

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

125447

Informations de copyright

Copyright © 2019 Elsevier Ltd. All rights reserved.

Auteurs

Yu-Chuan Wang (YC)

Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.

Hui-Fang Hu (HF)

Bioresource Utilization Laboratory, College of Engineering, China Agricultural University, Beijing 100083, China.

Jun-Wen Ma (JW)

Bioresource Utilization Laboratory, College of Engineering, China Agricultural University, Beijing 100083, China.

Qiao-Juan Yan (QJ)

Bioresource Utilization Laboratory, College of Engineering, China Agricultural University, Beijing 100083, China.

Hai-Jie Liu (HJ)

Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. Electronic address: liuhaijie@cau.edu.cn.

Zheng-Qiang Jiang (ZQ)

Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China. Electronic address: zhqjiang@cau.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Aliments et boissons › Aliments › Pain › A novel high maltose-forming α-amylase from...
questionsmedicales.fr › Technologie, industrie et agriculture › Industrie › Industrie alimentaire › A novel high maltose-forming α-amylase from...
questionsmedicales.fr › Phénomènes chimiques › Concentration en ions d'hydrogène › A novel high maltose-forming α-amylase from...
questionsmedicales.fr › Phénomènes chimiques › Hydrolyse › A novel high maltose-forming α-amylase from...
questionsmedicales.fr › Glucides › Sucres › Diholoside › Maltose › A novel high maltose-forming α-amylase from...
questionsmedicales.fr › Eucaryotes › Champignons › Levures › Pichia › A novel high maltose-forming α-amylase from...
questionsmedicales.fr › Eucaryotes › Champignons › Champignons non classés › Mucorales › Rhizomucor › A novel high maltose-forming α-amylase from...
questionsmedicales.fr › Glucides › Polyosides › Glucanes › Amidon › A novel high maltose-forming α-amylase from...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Spécificité du substrat › A novel high maltose-forming α-amylase from...
questionsmedicales.fr › Environnement et santé publique › Environnement › Concepts météorologiques › Atmosphère › Temps (météorologie) › Température › A novel high maltose-forming α-amylase from...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Glycosidases › Amylases › alpha-Amylases › A novel high maltose-forming α-amylase from...