Vaspin is a novel target gene of hepatic CCAAT-enhancer-binding protein.
C/EBP-responsive elements
CCAAT/enhancer binding protein
Liver
Vaspin
Journal
Gene
ISSN: 1879-0038
Titre abrégé: Gene
Pays: Netherlands
ID NLM: 7706761
Informations de publication
Date de publication:
30 Dec 2019
30 Dec 2019
Historique:
received:
16
06
2019
revised:
05
09
2019
accepted:
06
09
2019
pubmed:
11
9
2019
medline:
8
11
2019
entrez:
11
9
2019
Statut:
ppublish
Résumé
Vaspin, initially identified in visceral adipose tissue, is an adipokine, and administration of recombinant vaspin leads to lowering of the endoplasmic reticulum stress which is elevated in obesity or enhancement of insulin sensitivity. CCAAT/enhancer binding protein (C/EBP), as a basic leucine zipper transcription factor, plays a critical role in adipocyte development and glucose and lipid metabolisms in liver. The present study aimed to investigate the effect of C/EBPα on vaspin gene expression. The expression of hepatic vaspin was markedly decreased in liver-specific C/EBPα knockout mice. A reporter assay indicated that two C/EBP-responsive elements (CEBPREs) are necessary for C/EBPα-dependent induction of vaspin promoter activities. Furthermore, electrophoretic mobility shift assay showed that C/EBPα in mouse liver is capable of directly binding the two CEBPREs. These results suggest that C/EBPα positively regulates hepatic vaspin expression through two functional CEBPREs. Thus, vaspin is a novel C/EBPα target gene in the liver.
Identifiants
pubmed: 31505214
pii: S0378-1119(19)30772-3
doi: 10.1016/j.gene.2019.144113
pii:
doi:
Substances chimiques
Adipokines
0
CCAAT-Enhancer-Binding Proteins
0
CEBPA protein, mouse
0
Serpins
0
vaspin protein, mouse
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
144113Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.