Polyamine biosynthesis in Xenopus laevis: the xlAZIN2/xlODC2 gene encodes a lysine/ornithine decarboxylase.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2019
2019
Historique:
received:
30
05
2019
accepted:
26
08
2019
entrez:
12
9
2019
pubmed:
12
9
2019
medline:
10
3
2020
Statut:
epublish
Résumé
Ornithine decarboxylase (ODC) is a key enzyme in the biosynthesis of polyamines, organic cations that are implicated in many cellular processes. The enzyme is regulated at the post-translational level by an unusual system that includes antizymes (AZs) and antizyme inhibitors (AZINs). Most studies on this complex regulatory mechanism have been focused on human and rodent cells, showing that AZINs (AZIN1 and AZIN2) are homologues of ODC but devoid of enzymatic activity. Little is known about Xenopus ODC and its paralogues, in spite of the relevance of Xenopus as a model organism for biomedical research. We have used the information existing in different genomic databases to compare the functional properties of the amphibian ODC1, AZIN1 and AZIN2/ODC2, by means of transient transfection experiments of HEK293T cells. Whereas the properties of xlODC1 and xlAZIN1 were similar to those reported for their mammalian orthologues, the former catalyzing the decarboxylation of L-ornithine preferentially to that of L-lysine, xlAZIN2/xlODC2 showed important differences with respect to human and mouse AZIN2. xlAZIN2 did not behave as an antizyme inhibitor, but it rather acts as an authentic decarboxylase forming cadaverine, due to its higher affinity to L-lysine than to L-ornithine as substrate; so, in accordance with this, it should be named as lysine decarboxylase (LDC) or lysine/ornithine decarboxylase (LODC). In addition, AZ1 stimulated the degradation of xlAZIN2 by the proteasome, but the removal of the 21 amino acid C-terminal tail, with a sequence quite different to that of mouse or human ODC, made the protein resistant to degradation. Collectively, our results indicate that in Xenopus there is only one antizyme inhibitor (xlAZIN1) and two decarboxylases, xlODC1 and xlLDC, with clear preferences for L-ornithine and L-lysine, respectively.
Identifiants
pubmed: 31509528
doi: 10.1371/journal.pone.0218500
pii: PONE-D-19-15354
pmc: PMC6738921
doi:
Substances chimiques
Polyamines
0
RNA, Messenger
0
Carboxy-Lyases
EC 4.1.1.-
Ornithine Decarboxylase
EC 4.1.1.17
lysine decarboxylase
EC 4.1.1.18
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0218500Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Biochem Biophys Res Commun. 2000 Jan 7;267(1):1-6
pubmed: 10623564
Biochem Biophys Res Commun. 2000 May 19;271(3):559-64
pubmed: 10814501
Nat Rev Mol Cell Biol. 2001 Mar;2(3):188-94
pubmed: 11265248
Mech Dev. 2001 Apr;102(1-2):243-6
pubmed: 11287202
Proc Natl Acad Sci U S A. 2001 Sep 11;98(19):10620-3
pubmed: 11535806
EMBO J. 2003 Apr 1;22(7):1488-96
pubmed: 12660156
Nature. 1992 Dec 10;360(6404):597-9
pubmed: 1334232
J Biol Chem. 1992 Nov 15;267(32):23057-62
pubmed: 1429654
Nat Struct Mol Biol. 2004 Sep;11(9):830-7
pubmed: 15311270
J Biol Chem. 2004 Dec 24;279(52):54097-102
pubmed: 15491992
Nat Rev Cancer. 2004 Oct;4(10):781-92
pubmed: 15510159
Mol Cell Biol. 1992 May;12(5):2178-85
pubmed: 1569947
IUBMB Life. 2005 Oct;57(10):671-6
pubmed: 16223706
Mol Cell Biol. 1992 Aug;12(8):3556-62
pubmed: 1630460
J Biol Chem. 2006 May 26;281(21):14529-32
pubmed: 16459331
Cell Mol Life Sci. 2006 Sep;63(18):2095-101
pubmed: 16847581
J Biol Chem. 2006 Oct 13;281(41):30896-906
pubmed: 16916800
Mol Biol Evol. 2007 Jan;24(1):79-89
pubmed: 16997906
Eur J Biochem. 1991 Dec 5;202(2):575-81
pubmed: 1761057
Biochem J. 2008 Jan 1;409(1):187-92
pubmed: 17900240
Biochem J. 2008 Mar 15;410(3):613-9
pubmed: 18062773
J Biol Chem. 2008 Feb 22;283(8):4528-34
pubmed: 18089576
Int J Biochem Cell Biol. 2009 May;41(5):1070-8
pubmed: 18973822
Genes Cells. 2009 Jan;14(1):79-87
pubmed: 19077035
Cell Mol Life Sci. 2009 Aug;66(15):2479-88
pubmed: 19399584
J Cell Biochem. 2009 Jul 1;107(4):732-40
pubmed: 19449338
Int J Biochem Cell Biol. 2010 Jan;42(1):39-51
pubmed: 19643201
Amino Acids. 2010 Feb;38(2):603-11
pubmed: 19956990
Eur J Biochem. 1991 Mar 28;196(3):647-51
pubmed: 2013288
Biochim Biophys Acta. 1991 Apr 17;1092(2):161-4
pubmed: 2018782
J Mol Evol. 2010 Mar;70(3):289-302
pubmed: 20217058
Development. 1990 Nov;110(3):955-62
pubmed: 2088731
J Mol Biol. 2011 Apr 1;407(3):354-67
pubmed: 21295581
Amino Acids. 2012 Feb;42(2-3):539-47
pubmed: 21814789
Mol Cell. 2012 Nov 30;48(4):601-11
pubmed: 23041283
J Biol Chem. 1990 Jul 15;265(20):11823-6
pubmed: 2365702
Biochim Biophys Acta. 2014 Jan;1843(1):216-21
pubmed: 23684952
Biochim Biophys Acta. 2013 Nov;1830(11):5157-65
pubmed: 23872168
PLoS One. 2013 Jul 12;8(7):e69188
pubmed: 23874910
Gerontology. 2014;60(4):319-26
pubmed: 24481223
FEBS Open Bio. 2014 Jun 02;4:510-21
pubmed: 24967154
J Mol Biol. 2015 Oct 23;427(21):3389-406
pubmed: 26156863
Proc Natl Acad Sci U S A. 2015 Sep 8;112(36):11229-34
pubmed: 26305948
PLoS One. 2016 Mar 10;11(3):e0151175
pubmed: 26963840
J Biol Chem. 2016 Jul 15;291(29):14904-12
pubmed: 27268251
Biochem J. 2016 Dec 15;473(24):4551-4558
pubmed: 27941031
Biochem Biophys Res Commun. 1989 Jan 31;158(2):520-6
pubmed: 2917000
Science. 1989 Mar 17;243(4897):1493-5
pubmed: 2928784
Biochim Biophys Acta Gen Subj. 2018 Sep;1862(9):2053-2068
pubmed: 29890242
Med Sci (Basel). 2018 Oct 09;6(4):null
pubmed: 30304856
J Biol Chem. 2018 Nov 30;293(48):18730-18735
pubmed: 30355739
PLoS One. 2018 Dec 19;13(12):e0209202
pubmed: 30566531
FEBS Lett. 1979 Mar 15;99(2):325-8
pubmed: 428556
Biochim Biophys Acta. 1979 Jun 6;568(2):416-27
pubmed: 486492
J Exp Zool. 1983 Jul;227(1):121-6
pubmed: 6619761
Methods Enzymol. 1983;94:10-25
pubmed: 6621380
Endocrinol Jpn. 1981 Jun;28(3):305-12
pubmed: 6796394
Cell. 1995 Jan 13;80(1):51-60
pubmed: 7813017
Proc Natl Acad Sci U S A. 1994 Apr 26;91(9):3959-63
pubmed: 8171019
Int J Dev Biol. 1993 Dec;37(4):615-8
pubmed: 8180006
J Biol Chem. 1993 Nov 25;268(33):24572-9
pubmed: 8227016
Mol Cell Biol. 1993 Apr;13(4):2377-83
pubmed: 8455617
Biochemistry. 1993 Jun 8;32(22):5842-7
pubmed: 8504104