Normalization of organ bath contraction data for tissue specimen size: does one approach fit all?
Aorta
Cross-sectional area
Length
Normalization
Urinary bladder
Weight
Journal
Naunyn-Schmiedeberg's archives of pharmacology
ISSN: 1432-1912
Titre abrégé: Naunyn Schmiedebergs Arch Pharmacol
Pays: Germany
ID NLM: 0326264
Informations de publication
Date de publication:
02 2020
02 2020
Historique:
received:
24
07
2019
accepted:
02
09
2019
pubmed:
13
9
2019
medline:
5
1
2021
entrez:
13
9
2019
Statut:
ppublish
Résumé
Organ bath experiments are a key technology to assess contractility of smooth muscle. Despite efforts to standardize tissue specimen sizes, they vary to a certain degree. As it appears obvious that a larger piece of tissue should develop greater force, most investigators normalize contraction data for specimen size. However, they lack agreement which parameter should be used as denominator for normalization. A pre-planned analysis of data from a recent study was used to compare denominators used for normalization, i.e., weight, length, and cross-sectional area. To increase robustness, we compared force with denominator in correlation analysis and also coefficient of variation with different denominators. This was done concomitantly with urinary bladder strips and aortic rings and with multiple contractile stimuli. Our urinary bladder data show that normalization for strip weight yielded the tightest but still only moderate correlation (e.g., r
Identifiants
pubmed: 31511953
doi: 10.1007/s00210-019-01727-x
pii: 10.1007/s00210-019-01727-x
doi:
Substances chimiques
Phenylephrine
1WS297W6MV
Potassium Chloride
660YQ98I10
Carbachol
8Y164V895Y
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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