Differential expression of secretagogin immunostaining in the hippocampal formation and the entorhinal and perirhinal cortices of humans, rats, and mice.
RRID:AB_1079874
RRID:AB_10807945
comparative studies
entorhinal cortex
hippocampal formation
human, rat and mouse brains
immunostaining
perirhinal cortex
post-mortem
secretagogin
Journal
The Journal of comparative neurology
ISSN: 1096-9861
Titre abrégé: J Comp Neurol
Pays: United States
ID NLM: 0406041
Informations de publication
Date de publication:
01 03 2020
01 03 2020
Historique:
received:
11
06
2019
revised:
02
09
2019
accepted:
03
09
2019
pubmed:
13
9
2019
medline:
30
7
2021
entrez:
13
9
2019
Statut:
ppublish
Résumé
Secretagogin (SCGN) is a recently discovered calcium-binding protein belonging to the group of EF-hand calcium-binding proteins. SCGN immunostaining has been described in various regions of the human, rat and mouse brain. In these studies, it has been reported that, in general, the patterns of SCGN staining differ between rodents and human brains. These differences have been interpreted as uncovering phylogenetic differences in SCGN expression. Nevertheless, an important aspect that is not usually taken into account is that different methods are used for obtaining and processing brain tissue coming from humans and experimental animals. This is a critical issue since it has been shown that post-mortem time delay and the method of fixation (i.e., perfused vs. nonperfused brains) may influence the results of the immunostaining. Thus, it is not clear whether differences found in comparative studies with the human brain are simply due to technical factors or species-specific differences. In the present study, we analyzed the pattern of SCGN immunostaining in the adult human hippocampal formation (DG, CA1, CA2, CA3, subiculum, presubiculum, and parasubiculum) as well as in the entorhinal and perirhinal cortices. This pattern of immunostaining was compared with rat and mouse that were fixed either by perfusion or immersion and with different post-mortem time delays (up to 5 hr) to mimic the way the human brain tissue is usually processed. We found a number of clear similarities and differences in the pattern of labeling among the human, rat, and mouse in these brain regions as well as between the different brain regions examined within each species. These differences were not due to the fixation.
Identifiants
pubmed: 31512254
doi: 10.1002/cne.24773
pmc: PMC6972606
doi:
Substances chimiques
SCGN protein, human
0
SCGN protein, mouse
0
SCGN protein, rat
0
Secretagogins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
523-541Informations de copyright
© 2019 The Authors. The Journal of Comparative Neurology published by Wiley Periodicals, Inc.
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