Extensive ribosome and RF2 rearrangements during translation termination.

Cryoelectron Microscopy Escherichia coli / metabolism Escherichia coli Proteins / chemistry Peptide Chain Termination, Translational Peptide Termination Factors / chemistry Ribosomes / chemistry
E. coli GGQ motif rearrangement cell biology ensemble cryo-EM intersubunit rotation molecular biophysics release factor 2 ribosome recycling structural biology translation termination

Journal

eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614

Informations de publication

Date de publication:
12 09 2019
Historique:
received: 14 03 2019
accepted: 28 08 2019
entrez: 13 9 2019
pubmed: 13 9 2019
medline: 8 2 2020
Statut: epublish

Résumé

Protein synthesis ends when a ribosome reaches an mRNA stop codon. Release factors (RFs) decode the stop codon, hydrolyze peptidyl-tRNA to release the nascent protein, and then dissociate to allow ribosome recycling. To visualize termination by RF2, we resolved a cryo-EM ensemble of

Identifiants

DOI: 10.7554/eLife.46850 PMID: 31513010 PMC: PMC6742477
pubmed: 31513010
doi: 10.7554/eLife.46850
pii: 46850
pmc: PMC6742477
doi:
pii:

Substances chimiques

Escherichia coli Proteins 0
Peptide Termination Factors 0
prfB protein, E coli 0

Banques de données

PDB
['6OFX', '6OG7', '6OGF', '6OGI', '6OGG']

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NIGMS NIH HHS
ID : R35 GM127094
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM107465
Pays : United States

Informations de copyright

© 2019, Svidritskiy et al.

Déclaration de conflit d'intérêts

ES, GD, AL, CX, AK No competing interests declared

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Auteurs

Egor Svidritskiy (E)

RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, United States.

Gabriel Demo (G)

RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, United States.
ORCID: 0000-0002-5472-9249

Anna B Loveland (AB)

RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, United States.
ORCID: 0000-0001-9172-7747

Chen Xu (C)

Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, United States.

Andrei A Korostelev (AA)

RNA Therapeutics Institute, University of Massachusetts Medical School, Worcester, United States.
Biochemistry and Molecular Pharmacology, University of Massachusetts Medical School, Worcester, United States.
ORCID: 0000-0003-1588-717X

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Cryomicroscopie électronique › Extensive ribosome and RF2 rearrangements...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Extensive ribosome and RF2 rearrangements...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines bactériennes › Protéines Escherichia coli › Extensive ribosome and RF2 rearrangements...
questionsmedicales.fr › Métabolisme › Biosynthèse des peptides › Terminaison de la traduction › Extensive ribosome and RF2 rearrangements...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines ribosomiques › Facteurs terminaison chaîne peptidique › Extensive ribosome and RF2 rearrangements...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Organites › Ribosomes › Extensive ribosome and RF2 rearrangements...