Metastatic Breast Carcinoma-Associated Fibroblasts Have Enhanced Protumorigenic Properties Related to Increased IGF2 Expression.
Animals
Apoptosis
Breast Neoplasms
/ metabolism
Cancer-Associated Fibroblasts
/ metabolism
Cell Movement
Cell Proliferation
Epithelial-Mesenchymal Transition
Female
Humans
Insulin-Like Growth Factor II
/ metabolism
Mice
Mice, Nude
Neoplasm Invasiveness
Neoplasm Metastasis
Transcriptome
Tumor Cells, Cultured
Tumor Microenvironment
Xenograft Model Antitumor Assays
Journal
Clinical cancer research : an official journal of the American Association for Cancer Research
ISSN: 1557-3265
Titre abrégé: Clin Cancer Res
Pays: United States
ID NLM: 9502500
Informations de publication
Date de publication:
01 12 2019
01 12 2019
Historique:
received:
22
04
2019
revised:
05
07
2019
accepted:
29
08
2019
pubmed:
14
9
2019
medline:
22
9
2020
entrez:
14
9
2019
Statut:
ppublish
Résumé
The microenvironment of metastatic breast cancer is incompletely characterized, despite prior evidence that it plays a key role in the biology of metastasis. A major component of the tumor stroma is the carcinoma-associated fibroblast (CAF), which has been shown to communicate with other stromal and cancer cells to create a protumorigenic milieu. Our study was designed to characterize human CAFs from different metastatic sites. We collected eight carcinoma-associated fibroblasts (mCAFs) from different metastatic sites and compared them with CAFs from primary tumors (pCAFs) and with normal breast fibroblasts (NFs). Molecular profiles and effects on breast cancer cell growth, on response to doxorubicin and on T-cell proliferation were compared. We observed marked differences in mCAFs compared with pCAFs and NFs with respect to This is the first comparative analysis of a set of CAFs from metastatic sites in breast cancer. It revealed a marked protumorigenic effect in these mCAFs, which occurs in part through increased expression of IGF2.
Identifiants
pubmed: 31515454
pii: 1078-0432.CCR-19-1268
doi: 10.1158/1078-0432.CCR-19-1268
doi:
Substances chimiques
IGF2 protein, human
0
Insulin-Like Growth Factor II
67763-97-7
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
7229-7242Subventions
Organisme : CIHR
ID : FRN 53888
Pays : Canada
Informations de copyright
©2019 American Association for Cancer Research.