A chromosome-level genome assembly of Cydia pomonella provides insights into chemical ecology and insecticide resistance.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
17 09 2019
17 09 2019
Historique:
received:
08
02
2019
accepted:
20
08
2019
entrez:
19
9
2019
pubmed:
19
9
2019
medline:
7
1
2020
Statut:
epublish
Résumé
The codling moth Cydia pomonella, a major invasive pest of pome fruit, has spread around the globe in the last half century. We generated a chromosome-level scaffold assembly including the Z chromosome and a portion of the W chromosome. This assembly reveals the duplication of an olfactory receptor gene (OR3), which we demonstrate enhances the ability of C. pomonella to exploit kairomones and pheromones in locating both host plants and mates. Genome-wide association studies contrasting insecticide-resistant and susceptible strains identify hundreds of single nucleotide polymorphisms (SNPs) potentially associated with insecticide resistance, including three SNPs found in the promoter of CYP6B2. RNAi knockdown of CYP6B2 increases C. pomonella sensitivity to two insecticides, deltamethrin and azinphos methyl. The high-quality genome assembly of C. pomonella informs the genetic basis of its invasiveness, suggesting the codling moth has distinctive capabilities and adaptive potential that may explain its worldwide expansion.
Identifiants
pubmed: 31530873
doi: 10.1038/s41467-019-12175-9
pii: 10.1038/s41467-019-12175-9
pmc: PMC6748993
doi:
Substances chimiques
Insect Proteins
0
Insecticides
0
Pheromones
0
Receptors, Odorant
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4237Subventions
Organisme : Medical Research Council
ID : MR/K001744/1
Pays : United Kingdom
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