Imaging of the Axon Initial Segment.
Journal
Current protocols in neuroscience
ISSN: 1934-8576
Titre abrégé: Curr Protoc Neurosci
Pays: United States
ID NLM: 9706581
Informations de publication
Date de publication:
09 2019
09 2019
Historique:
entrez:
19
9
2019
pubmed:
19
9
2019
medline:
14
7
2020
Statut:
ppublish
Résumé
The axon initial segment (AIS) is the first 20- to 60-μm segment of the axon proximal to the soma of a neuron. This highly specialized subcellular domain is the initiation site of the action potential and contains a high concentration of voltage-gated ion channels held in place by a complex nexus of scaffolding and regulatory proteins that ensure proper electrical activity of the neuron. Studies have shown that dysfunction of many AIS channels and scaffolding proteins occurs in a variety of neuropsychiatric and neurodegenerative diseases, raising the need to develop accurate methods for visualization and quantification of the AIS and its protein content in models of normal and disease conditions. In this article, we describe methods for immunolabeling AIS proteins in cultured neurons and brain slices as well as methods for quantifying protein expression and pattern distribution using fluorescent labeling of these proteins. © 2019 by John Wiley & Sons, Inc.
Identifiants
pubmed: 31532918
doi: 10.1002/cpns.78
pmc: PMC6752718
mid: NIHMS1033517
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
e78Subventions
Organisme : NIMH NIH HHS
ID : R01 MH095995
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH111107
Pays : United States
Organisme : NIDA NIH HHS
ID : R01 DA047102
Pays : United States
Informations de copyright
© 2019 John Wiley & Sons, Inc.
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