A robust human norovirus replication model in zebrafish larvae.
Animals
Antiviral Agents
/ administration & dosage
Caliciviridae Infections
/ virology
Foodborne Diseases
/ virology
Gastroenteritis
/ virology
Host Microbial Interactions
Humans
Larva
/ virology
Metagenomics
Models, Animal
Norovirus
/ genetics
Virus Cultivation
/ methods
Virus Replication
/ drug effects
Zebrafish
/ virology
Journal
PLoS pathogens
ISSN: 1553-7374
Titre abrégé: PLoS Pathog
Pays: United States
ID NLM: 101238921
Informations de publication
Date de publication:
09 2019
09 2019
Historique:
received:
04
03
2019
accepted:
29
07
2019
entrez:
20
9
2019
pubmed:
20
9
2019
medline:
30
1
2020
Statut:
epublish
Résumé
Human noroviruses (HuNoVs) are the most common cause of foodborne illness, with a societal cost of $60 billion and 219,000 deaths/year. The lack of robust small animal models has significantly hindered the understanding of norovirus biology and the development of effective therapeutics. Here we report that HuNoV GI and GII replicate to high titers in zebrafish (Danio rerio) larvae; replication peaks at day 2 post infection and is detectable for at least 6 days. The virus (HuNoV GII.4) could be passaged from larva to larva two consecutive times. HuNoV is detected in cells of the hematopoietic lineage and the intestine, supporting the notion of a dual tropism. Antiviral treatment reduces HuNoV replication by >2 log10, showing that this model is suited for antiviral studies. Zebrafish larvae constitute a simple and robust replication model that will largely facilitate studies of HuNoV biology and the development of antiviral strategies.
Identifiants
pubmed: 31536612
doi: 10.1371/journal.ppat.1008009
pii: PPATHOGENS-D-19-00375
pmc: PMC6752765
doi:
Substances chimiques
Antiviral Agents
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1008009Subventions
Organisme : Wellcome Trust
ID : 097997/Z/11/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 207498/Z/17/Z
Pays : United Kingdom
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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