Generation of 3 clones of induced pluripotent stem cells (iPSCs) from a patient affected by Crohn's disease.
Journal
Stem cell research
ISSN: 1876-7753
Titre abrégé: Stem Cell Res
Pays: England
ID NLM: 101316957
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
received:
06
06
2019
revised:
09
08
2019
accepted:
21
08
2019
pubmed:
20
9
2019
medline:
7
5
2020
entrez:
20
9
2019
Statut:
ppublish
Résumé
Crohn's disease is a debilitating and incurable chronic inflammatory bowel disease, affecting millions of individuals worldwide, with an increasing frequency. Surgery must be applicable in half of the cases often with a disabling course, and pharmacological treatments may have adverse complications. We generated three isogenic clones of iPSCs from peripheral blood mononuclear cells (PBMCs) of a patient with Crohn's Disease under pharmacological treatment without adverse effects. Sendai virus based vector was used and the iPSCs were characterized for genetic uniqueness, genomic integrity, pluripotency, and differentiation ability. These iPSCs will be a powerful tool to develop tailored therapies.
Identifiants
pubmed: 31536868
pii: S1873-5061(19)30178-3
doi: 10.1016/j.scr.2019.101548
pii:
doi:
Substances chimiques
Octamer Transcription Factor-3
0
POU5F1 protein, human
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101548Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.