Characterization of drug resistance and genetic diversity of Plasmodium falciparum parasites from Tripura, Northeast India.


Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
23 09 2019
Historique:
received: 07 02 2019
accepted: 06 09 2019
entrez: 25 9 2019
pubmed: 25 9 2019
medline: 31 10 2020
Statut: epublish

Résumé

Monitoring of anti-malarial drug resistance is vital in Northeast India as this region shares its international border with Southeast Asia. Genetic diversity of Plasmodium parasites regulates transmission dynamics, disease severity and vaccine efficacy. P. falciparum chloroquine resistance transporter (Pfcrt), multidrug resistance-1 (Pfmdr-1) and kelch 13 propeller (PfK-13) genes which govern antimalarial drug resistance and three genetic diversity markers, merozoite surface protein 1 and 2 (Pfmsp-1, Pfmsp-2) and glutamate rich protein (Pfglurp) were evaluated from Tripura, Northeast India using molecular tools. In the Pfcrt gene, 87% isolates showed triple mutations at codons M74I, N75E and K76T. 12.5% isolates in Pfmdr-1 gene showed mutation at N86Y. No polymorphism in PfK-13 propeller was found. Polyclonal infections were observed in 53.85% isolates and more commonly in adults (p = 0.0494). In the Pfmsp-1 locus, the K1 allelic family was predominant (71.2%) followed by the 3D7/IC family (69.2%) in the Pfmsp-2 locus. RII region of Pfglurp exhibited nine alleles with expected heterozygosity of 0.85. The multiplicity of infection for Pfmsp-1, Pfmsp-2 and Pfglurp were 1.56, 1.31 and 1.06 respectively. Overall, the study demonstrated a high level of chloroquine resistance and extensive parasite diversity in the region, necessitating regular surveillance in this population group.

Identifiants

pubmed: 31548652
doi: 10.1038/s41598-019-50152-w
pii: 10.1038/s41598-019-50152-w
pmc: PMC6757058
doi:

Substances chimiques

Antimalarials 0
Chloroquine 886U3H6UFF

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

13704

Références

Malar J. 2016 Aug 15;15(1):414
pubmed: 27527604
PLoS One. 2013 Oct 09;8(10):e75064
pubmed: 24130682
Malar J. 2018 Apr 23;17(1):172
pubmed: 29685152
Trans R Soc Trop Med Hyg. 1999 Jul-Aug;93(4):369-74
pubmed: 10674079
Malar J. 2014 Nov 21;13:450
pubmed: 25416405
Korean J Parasitol. 2007 Mar;45(1):59-63
pubmed: 17374980
Am J Trop Med Hyg. 1999 Jan;60(1):30-4
pubmed: 9988318
Mol Cell. 2000 Oct;6(4):861-71
pubmed: 11090624
Sci Rep. 2017 Nov 30;7(1):16690
pubmed: 29192183
Trends Parasitol. 2012 Nov;28(11):504-14
pubmed: 23020971
Emerg Infect Dis. 2015 May;21(5):797-803
pubmed: 25897626
Emerg Infect Dis. 2007 Jun;13(6):872-7
pubmed: 17553226
Lancet. 2001 Sep 15;358(9285):890-1
pubmed: 11567708
Trans R Soc Trop Med Hyg. 1996 Nov-Dec;90(6):704-11
pubmed: 9015525
Indian J Med Res. 2017 Sep;146(3):375-380
pubmed: 29355145
PLoS Med. 2007 Mar;4(3):e93
pubmed: 17355170
Trop Med Int Health. 2017 Dec;22(12):1590-1598
pubmed: 29029367
Malar Res Treat. 2014;2014:950424
pubmed: 25506039
Malar J. 2016 Jul 11;15:353
pubmed: 27401016
Parasit Vectors. 2011 Dec 13;4:233
pubmed: 22166488
ScientificWorldJournal. 2014;2014:140867
pubmed: 25405214
Am J Trop Med Hyg. 2015 Sep;93(3 Suppl):57-68
pubmed: 26259943
J Infect Dis. 2011 Dec 1;204(11):1772-8
pubmed: 21998472
PLoS Negl Trop Dis. 2015 May 07;9(5):e0003739
pubmed: 25951184
PLoS One. 2017 Aug 7;12(8):e0182674
pubmed: 28787005
Malar J. 2016 Dec 3;15(1):583
pubmed: 27912758
Nature. 2014 Jan 2;505(7481):50-5
pubmed: 24352242
Indian J Med Microbiol. 2016 Apr-Jun;34(2):261-2
pubmed: 27080798
Methods Mol Med. 2002;72:103-16
pubmed: 12125106
PLoS Negl Trop Dis. 2016 Jan 11;10(1):e0004355
pubmed: 26751811
PLoS One. 2014 Sep 03;9(9):e105562
pubmed: 25184337
Indian J Med Res. 2014 Feb;139(2):205-15
pubmed: 24718394
Malar J. 2010 May 17;9:131
pubmed: 20478015
Trop Med Int Health. 2003 Jan;8(1):3-11
pubmed: 12535242
Acta Trop. 2005 Jun;94(3):181-90
pubmed: 15876420
J Infect Dis. 2003 Jun 15;187(12):1870-5
pubmed: 12792863
Exp Parasitol. 2003 May-Jun;104(1-2):14-9
pubmed: 12932754
J Pathog. 2015;2015:374354
pubmed: 26688755
Antimicrob Agents Chemother. 2015 May;59(5):2548-53
pubmed: 25691626
Malar J. 2012 Nov 22;11:386
pubmed: 23173674
J Genet. 2008 Apr;87(1):3-20
pubmed: 18560169
J Infect Dis. 2000 Mar;181(3):1206-9
pubmed: 10720557
Malar J. 2013 Oct 30;12:377
pubmed: 24172030
Bioinformatics. 2005 Jan 15;21(2):263-5
pubmed: 15297300
Trop Med Int Health. 2010 Jan;15(1):33-40
pubmed: 19912592
Malar J. 2009 May 18;8:106
pubmed: 19450282
Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7
pubmed: 271968
Mem Inst Oswaldo Cruz. 2011 Feb;106(1):78-84
pubmed: 21340360
Indian J Pathol Microbiol. 2005 Oct;48(4):429-38
pubmed: 16366089
Emerg Infect Dis. 2019 Jul;25(7):1330-1338
pubmed: 31211682
Am J Trop Med Hyg. 2004 Mar;70(3):238-44
pubmed: 15031510
Antimicrob Agents Chemother. 2019 Sep 23;63(10):
pubmed: 31332065
Nucleic Acids Res. 2012 Jul;40(Web Server issue):W597-603
pubmed: 22661580
J Infect Dis. 2018 Jun 5;218(1):35-43
pubmed: 29584918
Trop Med Int Health. 2002 May;7(5):395-408
pubmed: 12000649
J Infect Dis. 2001 Jul 15;184(2):237-41
pubmed: 11424024
Malar J. 2013 May 01;12:147
pubmed: 23634705
Trans R Soc Trop Med Hyg. 2005 May;99(5):389-95
pubmed: 15780346
J Mol Microbiol Biotechnol. 2007;13(1-3):89-95
pubmed: 17693716
Malar J. 2012 Jun 10;11:192
pubmed: 22682111
Mol Biochem Parasitol. 1993 Oct;61(2):315-20
pubmed: 8264734
Genom Data. 2016 Apr 18;8:85-90
pubmed: 27222806
Malar J. 2011 Oct 17;10:304
pubmed: 22004584
Vaccine. 2015 Dec 22;33(52):7433-43
pubmed: 26458807
Malar J. 2013 Jul 15;12:244
pubmed: 23855834
Infect Genet Evol. 2014 Aug;26:213-22
pubmed: 24911283

Auteurs

S J Patgiri (SJ)

ICMR - Regional Medical Research Centre, North East Region, Dibrugarh, Assam, India. saurav.patgiri@gmail.com.

K Sarma (K)

ICMR - Regional Medical Research Centre, North East Region, Dibrugarh, Assam, India.

N Sarmah (N)

ICMR - Regional Medical Research Centre, North East Region, Dibrugarh, Assam, India.
Model Rural Health Research Unit (DHR), Assam, India.

N Bhattacharyya (N)

ICMR - Regional Medical Research Centre, North East Region, Dibrugarh, Assam, India.

D K Sarma (DK)

ICMR - Regional Medical Research Centre, North East Region, Dibrugarh, Assam, India.
ICMR - National Institute for Research in Environmental Health, Bhopal, Madhya Pradesh, India.

T Nirmolia (T)

ICMR - Regional Medical Research Centre, North East Region, Dibrugarh, Assam, India.

D R Bhattacharyya (DR)

ICMR - Regional Medical Research Centre, North East Region, Dibrugarh, Assam, India.

P K Mohapatra (PK)

ICMR - Regional Medical Research Centre, North East Region, Dibrugarh, Assam, India.

D Bansal (D)

Department of Microbiology and Immunology, Weill Cornell Medicine-Qatar, Cornell University, Doha, Qatar.
Ministry of Public Health, Doha, Qatar.

P K Bharti (PK)

ICMR - National Institute for Research in Tribal Health, Jabalpur, Madhya Pradesh, India.

R Sehgal (R)

Department of Medical Parasitology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

J Mahanta (J)

ICMR - Regional Medical Research Centre, North East Region, Dibrugarh, Assam, India.

A A Sultan (AA)

Department of Microbiology and Immunology, Weill Cornell Medicine-Qatar, Cornell University, Doha, Qatar.

Articles similaires

T-Lymphocytes, Regulatory Lung Neoplasms Proto-Oncogene Proteins p21(ras) Animals Humans

Pathogenic mitochondrial DNA mutations inhibit melanoma metastasis.

Spencer D Shelton, Sara House, Luiza Martins Nascentes Melo et al.
1.00
DNA, Mitochondrial Humans Melanoma Mutation Neoplasm Metastasis

Prevalence and implications of fragile X premutation screening in Thailand.

Areerat Hnoonual, Sunita Kaewfai, Chanin Limwongse et al.
1.00
Humans Fragile X Mental Retardation Protein Thailand Male Female
Humans Receptors, Antigen, T-Cell Proto-Oncogene Proteins p21(ras) Pancreatic Neoplasms T-Lymphocytes

Classifications MeSH