CAR T cells targeting BAFF-R can overcome CD19 antigen loss in B cell malignancies.


Journal

Science translational medicine
ISSN: 1946-6242
Titre abrégé: Sci Transl Med
Pays: United States
ID NLM: 101505086

Informations de publication

Date de publication:
25 09 2019
Historique:
received: 07 02 2019
revised: 03 04 2019
accepted: 31 07 2019
entrez: 27 9 2019
pubmed: 27 9 2019
medline: 21 8 2020
Statut: ppublish

Résumé

CAR T cells targeting CD19 provide promising options for treatment of B cell malignancies. However, tumor relapse from antigen loss can limit efficacy. We developed humanized, second-generation CAR T cells against another B cell-specific marker, B cell activating factor receptor (BAFF-R), which demonstrated cytotoxicity against human lymphoma and acute lymphoblastic leukemia (ALL) lines. Adoptively transferred BAFF-R-CAR T cells eradicated 10-day preestablished tumor xenografts after a single treatment and retained efficacy against xenografts deficient in CD19 expression, including CD19-negative variants within a background of CD19-positive lymphoma cells. Four relapsed, primary ALLs with CD19 antigen loss obtained after CD19-directed therapy retained BAFF-R expression and activated BAFF-R-CAR, but not CD19-CAR, T cells. BAFF-R-CAR, but not CD19-CAR, T cells also demonstrated antitumor effects against an additional CD19 antigen loss primary patient-derived xenograft (PDX) in vivo. BAFF-R is amenable to CAR T cell therapy, and its targeting may prevent emergence of CD19 antigen loss variants.

Identifiants

pubmed: 31554741
pii: 11/511/eaaw9414
doi: 10.1126/scitranslmed.aaw9414
pmc: PMC7015136
mid: NIHMS1054892
pii:
doi:

Substances chimiques

Antigens, CD19 0
B-Cell Activation Factor Receptor 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NCI NIH HHS
ID : P30 CA016672
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA213138
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA157644
Pays : United States
Organisme : NCI NIH HHS
ID : R21 CA223141
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA137060
Pays : United States
Organisme : Howard Hughes Medical Institute
ID : 55108547
Pays : United States
Organisme : NCI NIH HHS
ID : R37 CA233691
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA033572
Pays : United States
Organisme : NCI NIH HHS
ID : R00 CA197498
Pays : United States
Organisme : NCI NIH HHS
ID : U10 CA180827
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA172558
Pays : United States
Organisme : NCI NIH HHS
ID : R35 CA197628
Pays : United States
Organisme : NCI NIH HHS
ID : P50 CA107399
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA236626
Pays : United States

Informations de copyright

Copyright © 2019 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.

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Auteurs

Hong Qin (H)

Toni Stephenson Lymphoma Center, Department of Hematology and Hematopoietic Cell Transplantation, Beckman Research Institute of City of Hope, Duarte, CA 91010, USA.

Zhenyuan Dong (Z)

Toni Stephenson Lymphoma Center, Department of Hematology and Hematopoietic Cell Transplantation, Beckman Research Institute of City of Hope, Duarte, CA 91010, USA.

Xiuli Wang (X)

Center for CAR T Cell Therapy, Department of Hematology and Hematopoietic Cell Transplantation, Beckman Research Institute of City of Hope, Duarte, CA 91010, USA.

Wesley A Cheng (WA)

Toni Stephenson Lymphoma Center, Department of Hematology and Hematopoietic Cell Transplantation, Beckman Research Institute of City of Hope, Duarte, CA 91010, USA.

Feng Wen (F)

Toni Stephenson Lymphoma Center, Department of Hematology and Hematopoietic Cell Transplantation, Beckman Research Institute of City of Hope, Duarte, CA 91010, USA.
Department of Medical Oncology Cancer Center, West China Hospital, Sichuan University, Sichuan 910041, China.

Weili Xue (W)

Toni Stephenson Lymphoma Center, Department of Hematology and Hematopoietic Cell Transplantation, Beckman Research Institute of City of Hope, Duarte, CA 91010, USA.
The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450001, China.

Han Sun (H)

Toni Stephenson Lymphoma Center, Department of Hematology and Hematopoietic Cell Transplantation, Beckman Research Institute of City of Hope, Duarte, CA 91010, USA.

Miriam Walter (M)

Center for CAR T Cell Therapy, Department of Hematology and Hematopoietic Cell Transplantation, Beckman Research Institute of City of Hope, Duarte, CA 91010, USA.

Guowei Wei (G)

Toni Stephenson Lymphoma Center, Department of Hematology and Hematopoietic Cell Transplantation, Beckman Research Institute of City of Hope, Duarte, CA 91010, USA.

D Lynne Smith (DL)

Toni Stephenson Lymphoma Center, Department of Hematology and Hematopoietic Cell Transplantation, Beckman Research Institute of City of Hope, Duarte, CA 91010, USA.

Xiuhua Sun (X)

The Second Affiliated Hospital of Dalian Medical University, Dalian 116044, China.

Fan Fei (F)

Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, CA 90007, USA.

Jianming Xie (J)

Department of Pharmacology and Pharmaceutical Sciences, School of Pharmacy, University of Southern California, CA 90007, USA.

Theano I Panagopoulou (TI)

Department of Systems Biology, Beckman Research Institute of City of Hope, Duarte, CA 91010, USA.

Chun-Wei Chen (CW)

Department of Systems Biology, Beckman Research Institute of City of Hope, Duarte, CA 91010, USA.

Joo Y Song (JY)

Department of Pathology, City of Hope National Medical Center, Duarte, CA 91010, USA.

Ibrahim Aldoss (I)

Gehr Family Center for Leukemia Research, Department of Hematology and Hematopoietic Cell Transplantation, Beckman Research Institute of City of Hope, Duarte, CA 91010, USA.

Clarisse Kayembe (C)

Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, NY 10065, USA.

Luisa Sarno (L)

Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, NY 10065, USA.

Markus Müschen (M)

Department of Systems Biology, Beckman Research Institute of City of Hope, Duarte, CA 91010, USA.

Giorgio G Inghirami (GG)

Department of Pathology and Laboratory Medicine, Weill Cornell Medical College, New York, NY 10065, USA.

Stephen J Forman (SJ)

Center for CAR T Cell Therapy, Department of Hematology and Hematopoietic Cell Transplantation, Beckman Research Institute of City of Hope, Duarte, CA 91010, USA.

Larry W Kwak (LW)

Toni Stephenson Lymphoma Center, Department of Hematology and Hematopoietic Cell Transplantation, Beckman Research Institute of City of Hope, Duarte, CA 91010, USA. lkwak@coh.org.

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