Simultaneous quantification of estrogens and glucocorticoids in human adipose tissue by liquid-chromatography-tandem mass spectrometry.
Adipose
Cortisol
Cortisone
Derivatization
Estradiol
Estrone
Journal
The Journal of steroid biochemistry and molecular biology
ISSN: 1879-1220
Titre abrégé: J Steroid Biochem Mol Biol
Pays: England
ID NLM: 9015483
Informations de publication
Date de publication:
12 2019
12 2019
Historique:
received:
21
03
2019
revised:
30
08
2019
accepted:
18
09
2019
pubmed:
29
9
2019
medline:
15
1
2020
entrez:
28
9
2019
Statut:
ppublish
Résumé
The presence of estrogens, androgens and glucocorticoids as well as their receptors and steroid converting enzymes in adipose tissue has been established. Their contribution to diseases such as obesity, diabetes and hormone-dependent cancers is an active area of research. Our objective was to develop a LC-MS/MS method to quantify bioactive estrogens and glucocorticoids simultaneously in human adipose tissue. Estrogens and glucocorticoids were extracted from adipose tissue samples using solid-phase extraction. Estrogens were derivatized using 1-(2,4-dinitro-5-fluorophenyl)-4-methylpiperazine (PPZ) and methyl iodide to generate a permanently charged molecule (MPPZ). Steroids were separated and quantified by LC-MS/MS. The limit of quantitation for the steroids was between 15 and 100 pg per sample. Accuracy and precision were acceptable (<20%). Using this method, estradiol, estrone, cortisone and cortisol were quantified in adipose tissue from women with and without breast cancer. This novel assay of estrogens and glucocorticoids by LC-MS/MS coupled with derivatization allowed simultaneous quantification of a panel of steroids in human adipose tissue across the endogenous range of concentrations encountered in health and disease.
Identifiants
pubmed: 31561001
pii: S0960-0760(19)30090-1
doi: 10.1016/j.jsbmb.2019.105476
pmc: PMC7099401
pii:
doi:
Substances chimiques
Estrogens
0
Glucocorticoids
0
Estrone
2DI9HA706A
Estradiol
4TI98Z838E
Cortisone
V27W9254FZ
Hydrocortisone
WI4X0X7BPJ
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
105476Subventions
Organisme : Medical Research Council
ID : MC_PC_12014
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/N503691/1
Pays : United Kingdom
Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier Ltd.. All rights reserved.
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