Laser phase plate for transmission electron microscopy.
Journal
Nature methods
ISSN: 1548-7105
Titre abrégé: Nat Methods
Pays: United States
ID NLM: 101215604
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
received:
14
12
2018
accepted:
06
08
2019
entrez:
29
9
2019
pubmed:
29
9
2019
medline:
15
2
2020
Statut:
ppublish
Résumé
Transmission electron microscopy (TEM) of rapidly frozen biological specimens, or cryo-EM, would benefit from the development of a phase plate for in-focus phase contrast imaging. Several types of phase plates have been investigated, but rapid electrostatic charging of all such devices has hindered these efforts. Here, we demonstrate electron phase manipulation with a high-intensity continuous-wave laser beam, and use it as a phase plate for TEM. We demonstrate the laser phase plate by imaging an amorphous carbon film. The laser phase plate provides a stable and tunable phase shift without electrostatic charging or unwanted electron scattering. These results suggest the possibility for dose-efficient imaging of unstained biological macromolecules and cells.
Identifiants
pubmed: 31562475
doi: 10.1038/s41592-019-0552-2
pii: 10.1038/s41592-019-0552-2
pmc: PMC6768090
mid: NIHMS1536822
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
1016-1020Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM126011
Pays : United States
Commentaires et corrections
Type : CommentIn
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