Towards the understanding of the UV light, riboflavin and additive solution contributions to the in vitro lesions observed in Mirasol®-treated platelets.
Blood Platelets
/ drug effects
Blood Preservation
/ methods
Blood Proteins
/ analysis
Blood Safety
Blood-Borne Pathogens
/ drug effects
Epinephrine
/ pharmacology
Humans
Organ Preservation Solutions
/ pharmacology
Osmotic Pressure
Phosphates
/ pharmacology
Photosensitizing Agents
/ pharmacology
Platelet Aggregation
/ drug effects
Platelet-Rich Plasma
Potassium Chloride
/ pharmacology
Riboflavin
/ pharmacology
Sodium
/ pharmacology
Sodium Acetate
/ pharmacology
Sodium Chloride
/ pharmacology
Sodium Citrate
/ pharmacology
Ultraviolet Rays
/ adverse effects
Additive solution
Inactivation des pathogènes
Lésion de stockage
Pathogen inactivation
Pathogen reduction
Platelet storage lesion
Riboflavin
Riboflavine
Réduction des pathogènes
Solution additive
Journal
Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine
ISSN: 1953-8022
Titre abrégé: Transfus Clin Biol
Pays: France
ID NLM: 9423846
Informations de publication
Date de publication:
Nov 2019
Nov 2019
Historique:
received:
18
06
2019
accepted:
07
09
2019
pubmed:
30
9
2019
medline:
5
6
2020
entrez:
30
9
2019
Statut:
ppublish
Résumé
Pathogen reduction technologies are implemented to increase the safety of blood products. We previously showed that the UVB alone significantly contributes to the storage lesions observed in platelets treated with riboflavin/UVB using a home-made illuminator. The present study aims at confirming these observations using the commercial Mirasol® technology. A three-arm study (untreated, UV-, Mirasol®-treated platelets) was conducted to investigate the platelet storage lesions throughout storage (n=4). A two-arm study was then designed to compare Intersol and T-PAS+ additive solutions (n=3). Phenotype and functional platelet characteristics were assessed using flow cytometry, aggregometry, antioxidant assays and metabolic parameters. Mirasol®-treated platelets exhibit enhanced storage lesions compared to controls (increase of activation markers and glycolysis rate, lower hypotonic shock and double-agonist activation responses, and decrease of total antioxidant capacity). Here, we also confirmed that the UV radiation alone is causing platelet lesions. Riboflavin tends to have an intracellular protective role while it decreases the extracellular antioxidant defenses. Furthermore, benefits of platelet additive solutions containing potassium and magnesium were confirmed as it reduces the extent of storage lesions. The photosensitizer, UV illumination and composition of the platelet additive solutions are key parameters influencing the platelet storage lesion. The clinical relevance of these findings is not fully understood and recent published clinical studies could not show increase in bleeding in patients receiving Mirasol-treated platelets. New developments in storage solutions might help to improve storage conditions of PRT-treated platelets and should be prioritised as research subject in the future.
Identifiants
pubmed: 31563447
pii: S1246-7820(19)30436-7
doi: 10.1016/j.tracli.2019.09.001
pii:
doi:
Substances chimiques
Blood Proteins
0
Organ Preservation Solutions
0
Phosphates
0
Photosensitizing Agents
0
Sodium Citrate
1Q73Q2JULR
Sodium Chloride
451W47IQ8X
Sodium Acetate
4550K0SC9B
Potassium Chloride
660YQ98I10
Sodium
9NEZ333N27
sodium phosphate
SE337SVY37
Riboflavin
TLM2976OFR
Epinephrine
YKH834O4BH
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
209-216Informations de copyright
Copyright © 2019 Société française de transfusion sanguine (SFTS). Published by Elsevier Masson SAS. All rights reserved.