Plasticity in exocytosis revealed through the effects of repetitive stimuli affect the content of nanometer vesicles and the fraction of transmitter released.
amperometry
exocytosis
plasticity
repetitive stimuli
vesicle impact electrochemical cytometry
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
22 10 2019
22 10 2019
Historique:
pubmed:
2
10
2019
medline:
1
4
2020
entrez:
2
10
2019
Statut:
ppublish
Résumé
Electrochemical techniques with disk and nano-tip electrodes, together with calcium imaging, were used to examine the effect of short-interval repetitive stimuli on both exocytosis and vesicular content in a model cell line. We show that the number of events decreases markedly with repeated stimuli suggesting a depletion of exocytosis machinery. However, repetitive stimuli induce a more stable fusion pore, leading to an increased amount of neurotransmitter release. In contrast, the total neurotransmitter content inside the vesicles decreases after repetitive stimuli, resulting in a higher average release fraction from each event. We suggest a possible mechanism regarding a link between activity-induced plasticity and fraction of release.
Identifiants
pubmed: 31570594
pii: 1910859116
doi: 10.1073/pnas.1910859116
pmc: PMC6815131
doi:
Substances chimiques
Neurotransmitter Agents
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
21409-21415Déclaration de conflit d'intérêts
The authors declare no competing interest.
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