Chemogenetic Control of Protein Anchoring to Endomembranes in Living Cells with Lipid-Tethered Small Molecules.
Cell Nucleus
/ metabolism
Endoplasmic Reticulum
/ metabolism
Escherichia coli
/ enzymology
Escherichia coli Proteins
/ metabolism
Extracellular Signal-Regulated MAP Kinases
/ metabolism
Golgi Apparatus
/ metabolism
HeLa Cells
Humans
Intracellular Membranes
/ metabolism
Ligands
Membrane Proteins
/ metabolism
Oleic Acids
/ pharmacology
Protein Transport
/ drug effects
Signal Transduction
/ drug effects
Tetrahydrofolate Dehydrogenase
/ metabolism
Trimethoprim
/ analogs & derivatives
ras Proteins
/ metabolism
Journal
Biochemistry
ISSN: 1520-4995
Titre abrégé: Biochemistry
Pays: United States
ID NLM: 0370623
Informations de publication
Date de publication:
21 01 2020
21 01 2020
Historique:
pubmed:
4
10
2019
medline:
14
7
2020
entrez:
4
10
2019
Statut:
ppublish
Résumé
The self-localizing ligand-induced protein translocation (SLIPT) system is an emerging platform that controls protein localization in living cells using synthetic self-localizing ligands (SLs). Here, we report a chemogenetic SLIPT system for inducing protein translocation from the cytoplasm to the surface of the endoplasmic reticulum (ER) and Golgi membranes, referred to as endomembranes. By screening a series of lipid-trimethoprim (TMP) conjugates, we found oleic acid-tethered TMP (oleTMP) to be the optimal SL that efficiently relocated and anchored
Identifiants
pubmed: 31578861
doi: 10.1021/acs.biochem.9b00807
doi:
Substances chimiques
Escherichia coli Proteins
0
Ligands
0
Membrane Proteins
0
Oleic Acids
0
Trimethoprim
AN164J8Y0X
Tetrahydrofolate Dehydrogenase
EC 1.5.1.3
Extracellular Signal-Regulated MAP Kinases
EC 2.7.11.24
ras Proteins
EC 3.6.5.2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM