Polyamine supplementation reduces DNA damage in adipose stem cells cultured in 3-D.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
03 10 2019
03 10 2019
Historique:
received:
15
01
2019
accepted:
10
09
2019
entrez:
5
10
2019
pubmed:
5
10
2019
medline:
3
11
2020
Statut:
epublish
Résumé
According to previous research, natural polyamines exert a role in regulating cell committment and differentiation from stemness during skeletal development. In order to assess whether distinct polyamine patterns are associated with different skeletal cell types, primary cultures of stem cells, chondrocytes or osteoblasts were dedicated for HPLC analysis of intracellular polyamines. Spermine (SPM) and Spermidine (SPD) levels were higher in adipose derived stem cells (ASC) compared to mature skeletal cells, i.e. chondrocytes and osteoblasts, confirming the connection of polyamine content with stemness. To establish whether polyamines can protect ASC against oxidative DNA damage in a 3-D differentiation model, the level of γH2AX was measured by western blot, and found to correlate with age and BMI of patients. Addition of either polyamine to ASC was able to hinder DNA damage in the low micromolecular range, with marked reduction of γH2AX level at 10 µM SPM and 5 µM SPD. Molecular analysis of the mechanisms that might underlie the protective effect of polyamine supplementation evidences a possible involvement of autophagy. Altogether, these results support the idea that polyamines are able to manage both stem cell differentiation and cell oxidative damage, and therefore represent appealing tools for regenerative and cell based applications.
Identifiants
pubmed: 31582764
doi: 10.1038/s41598-019-50543-z
pii: 10.1038/s41598-019-50543-z
pmc: PMC6776621
doi:
Substances chimiques
H2AX protein, human
0
Histones
0
Spermine
2FZ7Y3VOQX
Spermidine
U87FK77H25
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
14269Références
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