Kinetochore-associated Stu2 promotes chromosome biorientation in vivo.
Journal
PLoS genetics
ISSN: 1553-7404
Titre abrégé: PLoS Genet
Pays: United States
ID NLM: 101239074
Informations de publication
Date de publication:
10 2019
10 2019
Historique:
received:
04
09
2019
accepted:
13
09
2019
revised:
16
10
2019
pubmed:
5
10
2019
medline:
15
2
2020
entrez:
5
10
2019
Statut:
epublish
Résumé
Accurate segregation of chromosomes to daughter cells is a critical aspect of cell division. It requires the kinetochores on duplicated chromosomes to biorient, attaching to microtubules from opposite poles of the cell. Bioriented attachments come under tension, while incorrect attachments lack tension and must be released to allow proper attachments to form. A well-studied error correction pathway is mediated by the Aurora B kinase, which destabilizes low tension-bearing attachments. We recently discovered that in vitro, kinetochores display an additional intrinsic tension-sensing pathway that utilizes Stu2. The contribution of kinetochore-associated Stu2 to error correction in cells, however, was unknown. Here, we identify a Stu2 mutant that abolishes its kinetochore function and show that it causes biorientation defects in vivo. We also show that this Stu2-mediated pathway functions together with the Aurora B-mediated pathway. Altogether, our work indicates that cells employ multiple pathways to ensure biorientation and the accuracy of chromosome segregation.
Identifiants
pubmed: 31584935
doi: 10.1371/journal.pgen.1008423
pii: PGENETICS-D-19-01481
pmc: PMC6795502
doi:
Substances chimiques
Microtubule-Associated Proteins
0
STU2 protein, S cerevisiae
0
Saccharomyces cerevisiae Proteins
0
Aurora Kinases
EC 2.7.11.1
IPL1 protein, S cerevisiae
EC 2.7.11.1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1008423Subventions
Organisme : NIGMS NIH HHS
ID : P41 GM103533
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM098543
Pays : United States
Organisme : NIGMS NIH HHS
ID : P01 GM105537
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA015704
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM079373
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM040506
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM130293
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008297
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM064386
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM134842
Pays : United States
Organisme : NCI NIH HHS
ID : T32 CA080416
Pays : United States
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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