Anatomical and behavioral impact of a lentiviral tool tapping onto hippocampal serotonin reuptake in rats.
5-HT7 receptor
Porsolt's forced swim test
dentate gyrus
elevated plus maze (EPM)
in vivo gene silencing
magnetic resonance spectroscopy (1H-MRS)
novelty seeking test
serotonin transporter (SERT)
social interaction test (SIT)
Journal
Synapse (New York, N.Y.)
ISSN: 1098-2396
Titre abrégé: Synapse
Pays: United States
ID NLM: 8806914
Informations de publication
Date de publication:
04 2020
04 2020
Historique:
received:
16
07
2019
revised:
23
09
2019
accepted:
30
09
2019
pubmed:
7
10
2019
medline:
11
8
2021
entrez:
7
10
2019
Statut:
ppublish
Résumé
We aimed at the further characterization of rats in which SERT gene silencing was achieved by hippocampal injection of a lentiviral vector, carrying three si-RNA to block SERT mRNA at 66% of normal levels. Improved self-control and reduced restlessness were already demonstrated in these rats. Present further studies consisted of male adult rats, bilaterally inoculated within the hippocampus; control rats received lentivirus particles inactivated with heat. Both groups were maintained in isolation for 5 months, starting from inoculation. Neurochemical changes were studied by proton magnetic resonance spectroscopy (1H-MRS): we found increased hippocampal viability and bioenergetic potential; however, rats showed a behaviorally depressive pattern, also characterized by enhanced affiliation. Based on the extent of such effects, the whole lenti-SERT group was divided into two subgroups, termed intermediate- and extreme- phenotype profiles. While all rats had a widespread modification within dorsal/ventral striatum, amygdala, and hypothalamus, only the former subgroup showed an involvement of Raphé medialis, while, for the latter subgroup, an increase of SERT within hippocampus was unexpectedly caused. Within the less-affected "intermediate" rats, hippocampal 5-HT7 receptors were down-modulated, and also similarly within substantia nigra, septum, and neocortex. This picture demonstrates that additional rather than fewer neurobiological changes accompany a lower phenotypic expression. Overall, tapping hippocampal SERT affected the balance between habits versus strategies of coping by promoting morphogenetic processes indicative of a serotonergic fiber plasticity. Supplementary studies about serotonergic dynamics and neurogenesis within fronto-striatal circuits are needed.
Substances chimiques
RNA-Binding Proteins
0
Receptors, Serotonin
0
Sert1 protein, rat
0
serotonin 7 receptor
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e22138Informations de copyright
© 2019 Wiley Periodicals, Inc.
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