Resolving the fibrotic niche of human liver cirrhosis at single-cell level.

Animals Case-Control Studies Cell Lineage Duffy Blood-Group System / metabolism Endothelial Cells / metabolism Female Hepatic Stellate Cells / cytology Hepatocytes / cytology Humans Liver / cytology Liver Cirrhosis / genetics Macrophages / metabolism Male Membrane Glycoproteins / metabolism Membrane Proteins / metabolism Mice Phenotype Receptor, Platelet-Derived Growth Factor alpha / metabolism Receptors, Cell Surface / metabolism Receptors, Immunologic / metabolism Single-Cell Analysis Tetraspanin 29 / metabolism Transcriptome Transendothelial and Transepithelial Migration

Journal

Nature
ISSN: 1476-4687
Titre abrégé: Nature
Pays: England
ID NLM: 0410462

Informations de publication

Date de publication:
11 2019
Historique:
received: 04 09 2018
accepted: 04 09 2019
pubmed: 10 10 2019
medline: 22 4 2020
entrez: 10 10 2019
Statut: ppublish

Résumé

Liver cirrhosis is a major cause of death worldwide and is characterized by extensive fibrosis. There are currently no effective antifibrotic therapies available. To obtain a better understanding of the cellular and molecular mechanisms involved in disease pathogenesis and enable the discovery of therapeutic targets, here we profile the transcriptomes of more than 100,000 single human cells, yielding molecular definitions for non-parenchymal cell types that are found in healthy and cirrhotic human liver. We identify a scar-associated TREM2

Identifiants

DOI: 10.1038/s41586-019-1631-3 PMID: 31597160 PMC: PMC6876711
pubmed: 31597160
doi: 10.1038/s41586-019-1631-3
pii: 10.1038/s41586-019-1631-3
pmc: PMC6876711
mid: EMS84316
doi:

Substances chimiques

ACKR1 protein, human 0
Duffy Blood-Group System 0
Membrane Glycoproteins 0
Membrane Proteins 0
PLVAP protein, human 0
Receptors, Cell Surface 0
Receptors, Immunologic 0
TREM2 protein, human 0
Tetraspanin 29 0
Receptor, Platelet-Derived Growth Factor alpha EC 2.7.10.1

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

512-518

Subventions

Organisme : Medical Research Council
ID : MC_PC_17159
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_PC_15049
Pays : United Kingdom
Organisme : British Heart Foundation
ID : RM/17/3/33381
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_PC_15041
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/N022556/1
Pays : United Kingdom
Organisme : British Heart Foundation
ID : RE/18/5/34216
Pays : United Kingdom
Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : Medical Research Council
ID : G0600033
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/N008340/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : MR/P008887/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : G1002033
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_PC_15075
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UU_00007/15
Pays : United Kingdom
Organisme : Medical Research Council
ID : MC_UU_12008/1
Pays : United Kingdom
Organisme : Medical Research Council
ID : G84/6205
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 103749
Pays : United Kingdom

Commentaires et corrections

Type : CommentIn
Type : CommentIn

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Auteurs

P Ramachandran (P)

University of Edinburgh Centre for Inflammation Research, The Queen's Medical Research Institute, Edinburgh BioQuarter, Edinburgh, UK. prakash.ramachandran@ed.ac.uk.

R Dobie (R)

University of Edinburgh Centre for Inflammation Research, The Queen's Medical Research Institute, Edinburgh BioQuarter, Edinburgh, UK.

J R Wilson-Kanamori (JR)

University of Edinburgh Centre for Inflammation Research, The Queen's Medical Research Institute, Edinburgh BioQuarter, Edinburgh, UK.

E F Dora (EF)

University of Edinburgh Centre for Inflammation Research, The Queen's Medical Research Institute, Edinburgh BioQuarter, Edinburgh, UK.

B E P Henderson (BEP)

University of Edinburgh Centre for Inflammation Research, The Queen's Medical Research Institute, Edinburgh BioQuarter, Edinburgh, UK.

N T Luu (NT)

NIHR Birmingham Biomedical Research Centre, University Hospitals Birmingham NHS Foundation Trust and University of Birmingham, Birmingham, UK.
Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, UK.

J R Portman (JR)

University of Edinburgh Centre for Inflammation Research, The Queen's Medical Research Institute, Edinburgh BioQuarter, Edinburgh, UK.

K P Matchett (KP)

University of Edinburgh Centre for Inflammation Research, The Queen's Medical Research Institute, Edinburgh BioQuarter, Edinburgh, UK.

M Brice (M)

University of Edinburgh Centre for Inflammation Research, The Queen's Medical Research Institute, Edinburgh BioQuarter, Edinburgh, UK.

J A Marwick (JA)

University of Edinburgh Centre for Inflammation Research, The Queen's Medical Research Institute, Edinburgh BioQuarter, Edinburgh, UK.
Cancer Research UK Edinburgh Centre, MRC Institute of Genetics and Molecular Medicine at the University of Edinburgh, Edinburgh, UK.

R S Taylor (RS)

University of Edinburgh Centre for Inflammation Research, The Queen's Medical Research Institute, Edinburgh BioQuarter, Edinburgh, UK.

M Efremova (M)

Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.

R Vento-Tormo (R)

Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.

N O Carragher (NO)

Cancer Research UK Edinburgh Centre, MRC Institute of Genetics and Molecular Medicine at the University of Edinburgh, Edinburgh, UK.

T J Kendall (TJ)

University of Edinburgh Centre for Inflammation Research, The Queen's Medical Research Institute, Edinburgh BioQuarter, Edinburgh, UK.
Division of Pathology, University of Edinburgh, Edinburgh, UK.

J A Fallowfield (JA)

University of Edinburgh Centre for Inflammation Research, The Queen's Medical Research Institute, Edinburgh BioQuarter, Edinburgh, UK.

E M Harrison (EM)

Clinical Surgery, University of Edinburgh, Royal Infirmary of Edinburgh, Edinburgh, UK.

D J Mole (DJ)

University of Edinburgh Centre for Inflammation Research, The Queen's Medical Research Institute, Edinburgh BioQuarter, Edinburgh, UK.
Clinical Surgery, University of Edinburgh, Royal Infirmary of Edinburgh, Edinburgh, UK.

S J Wigmore (SJ)

University of Edinburgh Centre for Inflammation Research, The Queen's Medical Research Institute, Edinburgh BioQuarter, Edinburgh, UK.
Clinical Surgery, University of Edinburgh, Royal Infirmary of Edinburgh, Edinburgh, UK.

P N Newsome (PN)

NIHR Birmingham Biomedical Research Centre, University Hospitals Birmingham NHS Foundation Trust and University of Birmingham, Birmingham, UK.
Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, UK.

C J Weston (CJ)

NIHR Birmingham Biomedical Research Centre, University Hospitals Birmingham NHS Foundation Trust and University of Birmingham, Birmingham, UK.
Institute of Immunology and Immunotherapy, University of Birmingham, Birmingham, UK.

J P Iredale (JP)

Office of the Vice Chancellor, Beacon House and National Institute for Health Research, Biomedical Research Centre, Bristol, UK.

F Tacke (F)

Department of Hepatology and Gastroenterology, Charité University Medical Center, Berlin, Germany.

J W Pollard (JW)

MRC Centre for Reproductive Health, The Queen's Medical Research Institute, University of Edinburgh, Edinburgh, UK.
Department of Developmental and Molecular Biology, Albert Einstein College of Medicine, New York, NY, USA.

C P Ponting (CP)

MRC Human Genetics Unit, MRC Institute of Genetics and Molecular Medicine at the University of Edinburgh, Edinburgh, UK.

J C Marioni (JC)

Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.
European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Hinxton, Cambridge, UK.
Cancer Research UK Cambridge Institute, Li Ka Shing Centre, University of Cambridge, Cambridge, UK.

S A Teichmann (SA)

Wellcome Sanger Institute, Wellcome Genome Campus, Hinxton, Cambridge, UK.
European Molecular Biology Laboratory, European Bioinformatics Institute (EMBL-EBI), Hinxton, Cambridge, UK.
Theory of Condensed Matter Group, The Cavendish Laboratory, University of Cambridge, Cambridge, UK.

N C Henderson (NC)

University of Edinburgh Centre for Inflammation Research, The Queen's Medical Research Institute, Edinburgh BioQuarter, Edinburgh, UK. neil.henderson@ed.ac.uk.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Resolving the fibrotic niche of human liver...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Méthodes épidémiologiques › Caractéristiques des études épidémiologiques › Études épidémiologiques › Études cas-témoins › Resolving the fibrotic niche of human liver...
questionsmedicales.fr › Phénomènes physiologiques des appareils urinaire et reproducteur › Phénomènes physiologiques de la reproduction › Reproduction › Développement embryonnaire et foetal › Développement embryonnaire › Lignage cellulaire › Resolving the fibrotic niche of human liver...
questionsmedicales.fr › Facteurs biologiques › Antigènes › Isoantigènes › Antigènes de groupe sanguin › Système Duffy › Resolving the fibrotic niche of human liver...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules endothéliales › Resolving the fibrotic niche of human liver...
questionsmedicales.fr › Cellules › Cellules étoilées du foie › Resolving the fibrotic niche of human liver...
questionsmedicales.fr › Cellules › Cellules épithéliales › Hépatocytes › Resolving the fibrotic niche of human liver...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Resolving the fibrotic niche of human liver...
questionsmedicales.fr › Système digestif › Foie › Resolving the fibrotic niche of human liver...
questionsmedicales.fr › États, signes et symptômes pathologiques › Processus pathologiques › Fibrose › Cirrhose du foie › Resolving the fibrotic niche of human liver...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Phagocytes › Macrophages › Resolving the fibrotic niche of human liver...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Glycoprotéines membranaires › Resolving the fibrotic niche of human liver...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Resolving the fibrotic niche of human liver...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Souris › Resolving the fibrotic niche of human liver...
questionsmedicales.fr › Phénomènes génétiques › Phénotype › Resolving the fibrotic niche of human liver...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs peptidiques › Récepteur facteur croissance › Récepteurs aux facteurs de croissance dérivés des plaquettes › Récepteur au PDGF alpha › Resolving the fibrotic niche of human liver...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Resolving the fibrotic niche of human liver...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Récepteurs de surface cellulaire › Récepteurs immunologiques › Resolving the fibrotic niche of human liver...
questionsmedicales.fr › Techniques d'investigation › Techniques cytologiques › Analyse sur cellule unique › Resolving the fibrotic niche of human liver...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Tétraspanines › Antigène CD9 › Resolving the fibrotic niche of human liver...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Transcriptome › Resolving the fibrotic niche of human liver...
questionsmedicales.fr › Phénomènes physiologiques › Mouvement cellulaire › Migration transendothéliale et transépithéliale › Resolving the fibrotic niche of human liver...