Ultrasound Molecular Imaging of Atherosclerosis With Nanobodies: Translatable Microbubble Targeting Murine and Human VCAM (Vascular Cell Adhesion Molecule) 1.
Animals
Aorta, Thoracic
/ diagnostic imaging
Atherosclerosis
/ diagnosis
Brachiocephalic Trunk
/ diagnostic imaging
Cells, Cultured
Disease Models, Animal
Endothelial Cells
/ metabolism
Humans
Mice
Mice, Knockout
Microbubbles
Molecular Imaging
/ methods
Ultrasonography
/ methods
Vascular Cell Adhesion Molecule-1
/ biosynthesis
atherosclerosis
inflammation
microbubble
molecular imaging
ultrasonography
Journal
Arteriosclerosis, thrombosis, and vascular biology
ISSN: 1524-4636
Titre abrégé: Arterioscler Thromb Vasc Biol
Pays: United States
ID NLM: 9505803
Informations de publication
Date de publication:
12 2019
12 2019
Historique:
pubmed:
11
10
2019
medline:
14
4
2020
entrez:
11
10
2019
Statut:
ppublish
Résumé
Contrast-enhanced ultrasound molecular imaging (CEUMI) of endothelial expression of VCAM (vascular cell adhesion molecule)-1 could improve risk stratification for atherosclerosis. The microbubble contrast agents developed for preclinical studies are not suitable for clinical translation. Our aim was to characterize and validate a microbubble contrast agent using a clinically translatable single-variable domain immunoglobulin (nanobody) ligand. Approach and Results: Microbubble with a nanobody targeting VCAM-1 (MB CEUMI of the expression of VCAM-1 is feasible in murine models of atherosclerosis and on human tissue using a clinically translatable microbubble bearing a VCAM-1 targeted nanobody.
Identifiants
pubmed: 31597443
doi: 10.1161/ATVBAHA.119.313088
doi:
Substances chimiques
Vascular Cell Adhesion Molecule-1
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM