Metabarcoding of eukaryotic parasite communities describes diverse parasite assemblages spanning the primate phylogeny.
comparative method
gastrointestinal parasites
next generation sequencing
parasite communities
Journal
Molecular ecology resources
ISSN: 1755-0998
Titre abrégé: Mol Ecol Resour
Pays: England
ID NLM: 101465604
Informations de publication
Date de publication:
Jan 2020
Jan 2020
Historique:
received:
11
07
2019
revised:
02
10
2019
accepted:
07
10
2019
pubmed:
11
10
2019
medline:
2
6
2020
entrez:
11
10
2019
Statut:
ppublish
Résumé
Despite their ubiquity, in most cases little is known about the impact of eukaryotic parasites on their mammalian hosts. Comparative approaches provide a powerful method to investigate the impact of parasites on host ecology and evolution, though two issues are critical for such efforts: controlling for variation in methods of identifying parasites and incorporating heterogeneity in sampling effort across host species. To address these issues, there is a need for standardized methods to catalogue eukaryotic parasite diversity across broad phylogenetic host ranges. We demonstrate the feasibility of a metabarcoding approach for describing parasite communities by analysing faecal samples from 11 nonhuman primate species representing divergent lineages of the primate phylogeny and the full range of sampling effort (i.e. from no parasites reported in the literature to the best-studied primates). We detected a number of parasite families and regardless of prior sampling effort, metabarcoding of only ten faecal samples identified parasite families previously undescribed in each host (x̅ = 8.5 new families per species). We found more overlap between parasite families detected with metabarcoding and published literature when more research effort-measured as the number of publications-had been conducted on the host species' parasites. More closely related primates and those from the same continent had more similar parasite communities, highlighting the biological relevance of sampling even a small number of hosts. Collectively, results demonstrate that metabarcoding methods are sensitive and powerful enough to standardize studies of eukaryotic parasite communities across host species, providing essential new tools for macroecological studies of parasitism.
Identifiants
pubmed: 31600853
doi: 10.1111/1755-0998.13101
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
204-215Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : 1135/3-1
Organisme : Deutsche Forschungsgemeinschaft
ID : DE 1135/2-1
Organisme : Deutsche Forschungsgemeinschaft
ID : FI 929/7-1
Organisme : Deutsche Forschungsgemeinschaft
ID : FOR2136
Organisme : Deutsche Forschungsgemeinschaft
ID : KR 3834/5-1
Organisme : Deutsche Forschungsgemeinschaft
ID : Ka 1082-28-1
Organisme : Deutsche Forschungsgemeinschaft
ID : OS201/6-1
Organisme : Deutsche Forschungsgemeinschaft
ID : OS201/6-2
Organisme : Deutsche Forschungsgemeinschaft
ID : SCHU 1554/6-1
Organisme : Deutsche Forschungsgemeinschaft
ID : WI 2637/3-1
Organisme : Deutsche Forschungsgemeinschaft
ID : WI 2637/4-2
Organisme : Max Planck Society
Informations de copyright
© 2019 The Authors. Molecular Ecology Resources published by John Wiley & Sons Ltd.
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