[Effect of Quercetin on Wnt/β-Catenin Signal Pathway of K562 and K562R Cells].

To investigate the effect of Quercetin (Qu) on cell proliferation, apoptosis and cell cycle, as well as the expression changes of Wnt/β-catenin signaling pathway, apoptosis and cell cycle regulators and BCR-ABL in CML susceptible cells K562 and imatinib-resistant cells (IM) K562R. The trypan blue staining was used to detect the all proliferation. The cell cycle and apoptosis were detected by flow cytometry. The fluorescence quantitative PCR and Western blot were used to detect the expression of mRNA and protein respectively. After administration with 5, 10, 20, 40, 80, 160, 320 μmol/L Qu, the inhibition ratio in K562 cells was 5.07%, 5.98%, 11.09%, 31.88%, 56.89%, 70.44%, 86.63%; and that in K562R cells were 4.99%, 9.75%, 10.54%, 8.93%, 25.13%, 46.89%, 68.60%; IC Qu can inhibit the proliferation K562 and K562R cells, and decrease the drug resistance and increase the sensitivity, that relate with inhibiting Wnt/β-catenin signaling pathway, activating apoptosis pathway and cyclins. 槲皮素对K562和K562R细胞Wnt/β-catenin信号通路的影响. 观察槲皮素（Qu）对伊马替尼（IM）敏感细胞K562及耐药细胞K562R的增殖、凋亡、细胞周期的影响，探讨凋亡及细胞周期调控因子、Wnt/β-catenin信号通路及BCR-ABL的表达变化，为Qu的临床应用提供实验室依据. 应用台盼蓝染色法检测K562和K562R细胞的增殖水平；流式细胞术检测细胞凋亡及细胞周期的分布；荧光定量PCR及Western blot法分别检测Wnt/β-catenin信号通路成员、细胞凋亡及细胞周期相关因子的mRNA及蛋白的表达. 经Qu 5、10、20、40、80、160、320 μmol/L作用于K562细胞后，K562细胞抑制率分别为5.07%、5.98%、11.09%、31.88%、56.89%、70.44%、86.63%；K562R细胞抑制率分别为4.99%、9.75%、10.54%、8.93%、25.13%、46.89%、68.60%。K562、K562R的IC Qu能够抑制K562及K562R细胞的增殖，降低其耐药性，增加敏感性，这与Qu抑制Wnt/β-catenin信号通路、激活凋亡途径及细胞周期因子相关.