Proteomics Identification and Validation of Desmocollin-1 and Catechol-O-Methyltransferase as Proteins Associated with Breast Cancer Cell Migration and Metastasis.
Breast Neoplasms
/ genetics
Catechol O-Methyltransferase
/ genetics
Cell Line, Tumor
Cell Membrane
/ metabolism
Cell Movement
/ genetics
Desmocollins
/ genetics
Female
Gene Expression Regulation, Neoplastic
Humans
Lymphatic Metastasis
/ pathology
Neoplasm Invasiveness
Phenotype
Proteomics
Receptor, ErbB-2
Survival Analysis
Triple Negative Breast Neoplasms
/ genetics
Up-Regulation
/ genetics
breast cancer
mass spectrometry
metastasis
sequential window acquisition of all theoretical
transmembrane proteins
Journal
Proteomics
ISSN: 1615-9861
Titre abrégé: Proteomics
Pays: Germany
ID NLM: 101092707
Informations de publication
Date de publication:
11 2019
11 2019
Historique:
received:
31
03
2019
revised:
26
09
2019
pubmed:
17
10
2019
medline:
27
6
2020
entrez:
17
10
2019
Statut:
ppublish
Résumé
Biological treatment of many cancers currently targets membrane bound receptors located on a cell surface. To identify novel membrane proteins associated with migration and metastasis of breast cancer cells, a more migrating subpopulation of MDA-MB-231 breast cancer cell line is selected and characterized. A high-resolution quantitative mass spectrometry with SILAC labeling is applied to analyze their surfaceome and it is compared with that of parental MDA-MB-231 cells. Among 824 identified proteins (FDR < 0.01), 128 differentially abundant cell surface proteins with at least one transmembrane domain are found. Of these, i) desmocollin-1 (DSC1) is validated as a protein connected with lymph node status of luminal A breast cancer, tumor grade, and Her-2 status by immunohistochemistry in the set of 96 primary breast tumors, and ii) catechol-O-methyltransferase is successfully verified as a protein associated with lymph node metastasis of triple negative breast cancer as well as with tumor grade by targeted data extraction from the SWATH-MS data of the same set of tissues. The findings indicate importance of both proteins for breast cancer development and metastasis and highlight the potential of biomarker validation strategy via targeted data extraction from SWATH-MS datasets.
Identifiants
pubmed: 31617665
doi: 10.1002/pmic.201900073
doi:
Substances chimiques
DSC1 protein, human
0
Desmocollins
0
COMT protein, human
EC 2.1.1.6
Catechol O-Methyltransferase
EC 2.1.1.6
ERBB2 protein, human
EC 2.7.10.1
Receptor, ErbB-2
EC 2.7.10.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e1900073Informations de copyright
© 2019 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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