Clonality analysis of pulmonary tumors by genome-wide copy number profiling.


Journal

PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081

Informations de publication

Date de publication:
2019
Historique:
received: 14 01 2019
accepted: 30 09 2019
entrez: 17 10 2019
pubmed: 17 10 2019
medline: 17 3 2020
Statut: epublish

Résumé

Multiple tumors in patients are frequently diagnosed, either synchronous or metachronous. The distinction between a second primary and a metastasis is important for treatment. Chromosomal DNA copy number aberrations (CNA) patterns are highly unique to specific tumors. The aim of this study was to assess genome-wide CNA-patterns as method to identify clonally related tumors in a prospective cohort of patients with synchronous or metachronous tumors, with at least one intrapulmonary tumor. In total, 139 tumor pairs from 90 patients were examined: 35 synchronous and 104 metachronous pairs. Results of CNA were compared to histological type, clinicopathological methods (Martini-Melamed-classification (MM) and ACCP-2013-criteria), and, if available, EGFR- and KRAS-mutation analysis. CNA-results were clonal in 74 pairs (53%), non-clonal in 33 pairs (24%), and inconclusive in 32 pairs (23%). Histological similarity was found in 130 pairs (94%). Concordance between histology and conclusive CNA-results was 69% (74 of 107 pairs: 72 clonal and two non-clonal). In 31 of 103 pairs with similar histology, genetics revealed non-clonality. In two out of four pairs with non-matching histology, genetics revealed clonality. The subgroups of synchronous and metachronous pairs showed similar outcome for the comparison of histological versus CNA-results. MM-classification and ACCP-2013-criteria, applicable on 34 pairs, and CNA-results were concordant in 50% and 62% respectively. Concordance between mutation matching and conclusive CNA-results was 89% (8 of 9 pairs: six clonal and two non-clonal). Interestingly, in one patient both tumors had the same KRAS mutation, but the CNA result was non-clonal. In conclusion, although some concordance between histological comparison and CNA profiling is present, arguments exist to prefer extensive molecular testing to determine whether a second tumor is a metastasis or a second primary.

Identifiants

pubmed: 31618260
doi: 10.1371/journal.pone.0223827
pii: PONE-D-19-01280
pmc: PMC6795528
doi:

Substances chimiques

KRAS protein, human 0
EGFR protein, human EC 2.7.10.1
ErbB Receptors EC 2.7.10.1
Proto-Oncogene Proteins p21(ras) EC 3.6.5.2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0223827

Commentaires et corrections

Type : ErratumIn

Déclaration de conflit d'intérêts

The authors have declared that no competing interests exist.

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Auteurs

Julien P L Vincenten (JPL)

Amsterdam UMC, location VUmc, Department of Pulmonary Diseases, Amsterdam, The Netherlands.
Albert Schweitzer Hospital, Department of Pulmonary Diseases, Dordrecht, The Netherlands.

Hendrik F van Essen (HF)

Amsterdam UMC, location VUmc, Tumor Genome Analysis Core, Cancer Center Amsterdam, The Netherlands.

Birgit I Lissenberg-Witte (BI)

Amsterdam UMC, location VUmc, Department of Epidemiology and Biostatistics, Amsterdam, The Netherlands.

Nicole W J Bulkmans (NWJ)

Spaarne Gasthuis, Department of Pathology, Haarlem, The Netherlands.

Oscar Krijgsman (O)

Netherlands Cancer Institute - Antoni van Leeuwenhoek, Department of Molecular Oncology & Immunology, Amsterdam, The Netherlands.

Daoud Sie (D)

Amsterdam UMC, location VUmc, Tumor Genome Analysis Core, Cancer Center Amsterdam, The Netherlands.

Paul P Eijk (PP)

Amsterdam UMC, location VUmc, Tumor Genome Analysis Core, Cancer Center Amsterdam, The Netherlands.

Egbert F Smit (EF)

Amsterdam UMC, location VUmc, Department of Pulmonary Diseases, Amsterdam, The Netherlands.
Netherlands Cancer Institute - Antoni van Leeuwenhoek, Department of Thoracic Oncology, Amsterdam, The Netherlands.

Bauke Ylstra (B)

Amsterdam UMC, location VUmc, Tumor Genome Analysis Core, Cancer Center Amsterdam, The Netherlands.

Erik Thunnissen (E)

Amsterdam UMC, location VUmc, Department of Pathology, Amsterdam, The Netherlands.

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