Tumor-infiltrating B cells affect the progression of oropharyngeal squamous cell carcinoma via cell-to-cell interactions with CD8


Journal

Journal for immunotherapy of cancer
ISSN: 2051-1426
Titre abrégé: J Immunother Cancer
Pays: England
ID NLM: 101620585

Informations de publication

Date de publication:
17 10 2019
Historique:
received: 15 05 2019
accepted: 30 08 2019
entrez: 19 10 2019
pubmed: 19 10 2019
medline: 4 8 2020
Statut: epublish

Résumé

Standard treatment of oropharyngeal squamous cell carcinoma (OPSCC) is associated with high morbidity, whereas immunotherapeutic approaches using PD-1:PD-L1 checkpoint blockade only show moderate response rates in OPSCC patients. Therefore, a better stratification of patients and the development of novel therapeutic protocols are crucially needed. The importance of tumor-infiltrating B cells (TIL-Bs) in shaping antitumor immunity remains unclear; therefore, we analyzed frequency, phenotype, prognostic value and possible roles of TIL-Bs in OPSCC. We utilized transcriptomic analysis of immune response-related genes in 18 OPSCC samples with respect to human papillomavirus (HPV) status. The density and localization of CD20 We observed significantly higher expression of B cell-related genes and higher densities of CD20 Our results indicate that high abundance of TIL-Bs and high density of direct B cell/CD8

Sections du résumé

BACKGROUND
Standard treatment of oropharyngeal squamous cell carcinoma (OPSCC) is associated with high morbidity, whereas immunotherapeutic approaches using PD-1:PD-L1 checkpoint blockade only show moderate response rates in OPSCC patients. Therefore, a better stratification of patients and the development of novel therapeutic protocols are crucially needed. The importance of tumor-infiltrating B cells (TIL-Bs) in shaping antitumor immunity remains unclear; therefore, we analyzed frequency, phenotype, prognostic value and possible roles of TIL-Bs in OPSCC.
METHODS
We utilized transcriptomic analysis of immune response-related genes in 18 OPSCC samples with respect to human papillomavirus (HPV) status. The density and localization of CD20
RESULTS
We observed significantly higher expression of B cell-related genes and higher densities of CD20
CONCLUSIONS
Our results indicate that high abundance of TIL-Bs and high density of direct B cell/CD8

Identifiants

pubmed: 31623665
doi: 10.1186/s40425-019-0726-6
pii: 10.1186/s40425-019-0726-6
pmc: PMC6796441
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

261

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Auteurs

Kamila Hladíková (K)

SOTIO a.s, Jankovcova 1518/2, CZ-17000 Prague 7, Prague, Czech Republic.
Department of Immunology, 2nd Faculty of Medicine, Charles University and University Hospital Motol, Prague, Czech Republic.

Vladimír Koucký (V)

SOTIO a.s, Jankovcova 1518/2, CZ-17000 Prague 7, Prague, Czech Republic.
Department of Otorhinolaryngology and Head and Neck Surgery, 1st Faculty of Medicine, Charles University and University Hospital Motol, Prague, Czech Republic.

Jan Bouček (J)

Department of Otorhinolaryngology and Head and Neck Surgery, 1st Faculty of Medicine, Charles University and University Hospital Motol, Prague, Czech Republic.

Jan Laco (J)

The Fingerland Department of Pathology, Charles University Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Hradec Králové, Czech Republic.

Marek Grega (M)

Department of Pathology and Molecular Medicine, 2nd Faculty of Medicine, Charles University and University Hospital Motol, Prague, Czech Republic.

Miroslav Hodek (M)

Department of Oncology and Radiotherapy, Charles University Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Hradec Králové, Czech Republic.

Michal Zábrodský (M)

Department of Otorhinolaryngology and Head and Neck Surgery, 1st Faculty of Medicine, Charles University and University Hospital Motol, Prague, Czech Republic.

Milan Vošmik (M)

Department of Oncology and Radiotherapy, Charles University Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Hradec Králové, Czech Republic.

Kateřina Rozkošová (K)

The Fingerland Department of Pathology, Charles University Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Hradec Králové, Czech Republic.

Hana Vošmiková (H)

The Fingerland Department of Pathology, Charles University Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Hradec Králové, Czech Republic.

Petr Čelakovský (P)

Department of Otorhinolaryngology and Head and Neck Surgery, Charles University Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Hradec Králové, Czech Republic.

Viktor Chrobok (V)

Department of Otorhinolaryngology and Head and Neck Surgery, Charles University Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Hradec Králové, Czech Republic.

Aleš Ryška (A)

The Fingerland Department of Pathology, Charles University Faculty of Medicine in Hradec Králové and University Hospital Hradec Králové, Hradec Králové, Czech Republic.

Radek Špíšek (R)

SOTIO a.s, Jankovcova 1518/2, CZ-17000 Prague 7, Prague, Czech Republic.

Anna Fialová (A)

SOTIO a.s, Jankovcova 1518/2, CZ-17000 Prague 7, Prague, Czech Republic. fialova@sotio.com.

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