Optimizing cell encapsulation condition in ECM-Collagen I hydrogels to support 3D neuronal cultures.

Cell Encapsulation / methods Cerebral Cortex / cytology Collagen Type I Extracellular Matrix Humans Hydrogels Neurons / cytology Primary Cell Culture / methods
3D Neuronal culture Cortical neurons ECM-collagen hydrogel Entrapment Hydrogel

Journal

Journal of neuroscience methods
ISSN: 1872-678X
Titre abrégé: J Neurosci Methods
Pays: Netherlands
ID NLM: 7905558

Informations de publication

Date de publication:
01 01 2020
Historique:
received: 29 08 2019
revised: 30 09 2019
accepted: 07 10 2019
pubmed: 19 10 2019
medline: 16 3 2021
entrez: 19 10 2019
Statut: ppublish

Résumé

The emergence of three-dimensional (3D) cell culture in neural tissue engineering has significantly elevated the complexity and relevance of in vitro systems. This is due in large part to the incorporation of biomaterials to impart structural dimensionality on the neuronal cultures. However, a comprehensive understanding of how key seeding parameters affect changes in cell distribution and viability remain unreported. In this study, we systematically evaluated permutations in seeding conditions (i.e., cell concentration and atmospheric CO At 14 days in vitro (DIV), cell distribution within the hydrogel was dependent on cell concentration and atmospheric CO ECM-Collagen gels have been shown to increase cell viability of neurons long-term. In using ECM-collagen gels, we highlight the importance of optimizing seeding parameters and thorough 3D culture characterization to understand the neurophysiological responses of these 3D systems.

Sections du résumé

BACKGROUND
The emergence of three-dimensional (3D) cell culture in neural tissue engineering has significantly elevated the complexity and relevance of in vitro systems. This is due in large part to the incorporation of biomaterials to impart structural dimensionality on the neuronal cultures. However, a comprehensive understanding of how key seeding parameters affect changes in cell distribution and viability remain unreported.
NEW METHOD
In this study, we systematically evaluated permutations in seeding conditions (i.e., cell concentration and atmospheric CO
RESULTS
At 14 days in vitro (DIV), cell distribution within the hydrogel was dependent on cell concentration and atmospheric CO
COMPARISON WITH EXISTING METHODS
ECM-Collagen gels have been shown to increase cell viability of neurons long-term.
CONCLUSION
In using ECM-collagen gels, we highlight the importance of optimizing seeding parameters and thorough 3D culture characterization to understand the neurophysiological responses of these 3D systems.

Identifiants

DOI: 10.1016/j.jneumeth.2019.108460 PMID: 31626846
pubmed: 31626846
pii: S0165-0270(19)30317-6
doi: 10.1016/j.jneumeth.2019.108460
pii:
doi:

Substances chimiques

Collagen Type I 0
Hydrogels 0

Types de publication

Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

108460

Informations de copyright

Copyright © 2019 The Authors. Published by Elsevier B.V. All rights reserved.

Auteurs

Doris Lam (D)

Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA.

Heather A Enright (HA)

Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA.

Sandra K G Peters (SKG)

Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA.

Monica L Moya (ML)

Engineering Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA.

David A Soscia (DA)

Engineering Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA.

Jose Cadena (J)

Engineering Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA.

Javier A Alvarado (JA)

Engineering Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA.

Kristen S Kulp (KS)

Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA.

Elizabeth K Wheeler (EK)

Engineering Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA.

Nicholas O Fischer (NO)

Physical and Life Sciences Directorate, Lawrence Livermore National Laboratory, Livermore, CA, USA. Electronic address: fischer29@llnl.gov.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques in vitro › Techniques de culture › Encapsulation de cellules › Optimizing cell encapsulation condition in...
questionsmedicales.fr › Système nerveux › Système nerveux central › Encéphale › Prosencéphale › Télencéphale › Cerveau › Cortex cérébral › Optimizing cell encapsulation condition in...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Scléroprotéines › Protéines de la matrice extracellulaire › Collagène › Collagènes fibrillaires › Collagène de type I › Optimizing cell encapsulation condition in...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace extracellulaire › Matrice extracellulaire › Optimizing cell encapsulation condition in...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Optimizing cell encapsulation condition in...
questionsmedicales.fr › Préparations pharmaceutiques › Colloïdes › Gels › Hydrogels › Optimizing cell encapsulation condition in...
questionsmedicales.fr › Cellules › Neurones › Optimizing cell encapsulation condition in...
questionsmedicales.fr › Techniques d'investigation › Techniques in vitro › Techniques de culture › Techniques de culture cellulaire › Culture de cellules primaires › Optimizing cell encapsulation condition in...