Functional Characterization of CD11c
Journal
Journal of immunology (Baltimore, Md. : 1950)
ISSN: 1550-6606
Titre abrégé: J Immunol
Pays: United States
ID NLM: 2985117R
Informations de publication
Date de publication:
01 12 2019
01 12 2019
Historique:
received:
08
04
2019
accepted:
24
09
2019
pubmed:
23
10
2019
medline:
28
5
2020
entrez:
23
10
2019
Statut:
ppublish
Résumé
Age-associated B cells (ABCs) are a unique subset of B cells defined by surface CD11b and CD11c expression. Although ABC expansion has been observed in both human and animal studies in the setting of advanced age, during humoral autoimmunity and following viral infection, the functional properties of this cellular subset remain incompletely defined. In the current study, we demonstrate that ABCs fulfill the criteria for memory B cells (MBCs), based on evidence of Ag-dependent expansion and persistence in a state poised for rapid differentiation into Ab-secreting plasma cells during secondary responses. First, we show that a majority of ABCs are not actively cycling but exhibit an extensive replication history consistent with prior Ag engagement. Second, despite unswitched surface IgM expression, ABCs show evidence of activation-induced cytidine deaminase (AID)-dependent somatic hypermutation. Third, BCRs cloned from sorted ABCs exhibit broad autoreactivity and polyreactivity. Although the overall level of ABC self-reactivity was not increased relative to naive B cells, ABCs lacked features of functional anergy characteristic of autoreactive B cells. Fourth, ABCs express MBC surface markers consistent with being poised for rapid plasma cell differentiation during recall responses. Finally, in a murine model of viral infection, adoptively transferred CD11c
Identifiants
pubmed: 31636237
pii: jimmunol.1900404
doi: 10.4049/jimmunol.1900404
pmc: PMC6864310
mid: NIHMS1540751
doi:
Substances chimiques
CD11c Antigen
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2817-2826Subventions
Organisme : NIAID NIH HHS
ID : K08 AI112993
Pays : United States
Organisme : NHLBI NIH HHS
ID : P01 HL053749
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR073938
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI123818
Pays : United States
Organisme : NIDDK NIH HHS
ID : DP3 DK111802
Pays : United States
Informations de copyright
Copyright © 2019 by The American Association of Immunologists, Inc.
Références
J Immunol. 2016 Aug 15;197(4):1023-8
pubmed: 27430719
Eur J Immunol. 2015 Oct;45(10):2773-9
pubmed: 26256668
JCI Insight. 2017 Jun 15;2(12):null
pubmed: 28614800
J Immunol. 2017 Oct 1;199(7):2249-2260
pubmed: 28801357
J Exp Med. 2011 Sep 26;208(10):2033-42
pubmed: 21875954
Nat Neurosci. 2010 Jan;13(1):133-40
pubmed: 20023653
J Immunol. 2014 May 15;192(10):4525-32
pubmed: 24711620
J Clin Invest. 2001 Oct;108(7):1061-70
pubmed: 11581307
Blood. 2011 Aug 4;118(5):1294-304
pubmed: 21562046
J Immunol. 2009 Aug 1;183(3):2176-82
pubmed: 19592645
Eur J Immunol. 2018 Mar;48(3):509-521
pubmed: 29266242
PLoS Pathog. 2016 Jun 15;12(6):e1005687
pubmed: 27304615
J Immunol. 2013 Aug 1;191(3):1240-9
pubmed: 23804710
Immunity. 2011 Mar 25;34(3):375-84
pubmed: 21353603
Nat Immunol. 2015 Jul;16(7):755-65
pubmed: 26006014
J Exp Med. 2007 Sep 3;204(9):2103-14
pubmed: 17698588
J Exp Med. 2005 Mar 7;201(5):703-11
pubmed: 15738055
Annu Rev Immunol. 2017 Apr 26;35:255-284
pubmed: 28142324
Clin Exp Immunol. 2007 Jan;147(1):60-70
pubmed: 17177964
J Clin Invest. 2013 May;123(5):2024-36
pubmed: 23619366
Annu Rev Immunol. 1994;12:487-520
pubmed: 8011289
J Immunol Methods. 2014 Jun;408:89-100
pubmed: 24857755
Blood. 2011 Aug 4;118(5):1305-15
pubmed: 21543762
Cell Rep. 2018 Jul 24;24(4):824-837.e3
pubmed: 30044980
Science. 2003 Sep 5;301(5638):1374-7
pubmed: 12920303
J Immunol. 2007 Sep 1;179(5):3351-61
pubmed: 17709552
Cell Immunol. 2017 Nov;321:40-45
pubmed: 28756897
Nat Immunol. 2014 Jul;15(7):631-7
pubmed: 24880458
Science. 2011 Mar 4;331(6021):1203-7
pubmed: 21310965
Blood. 2010 Jun 17;115(24):5026-36
pubmed: 20231422
J Exp Med. 2007 Mar 19;204(3):645-55
pubmed: 17312005
PLoS One. 2011 Jan 14;6(1):e15983
pubmed: 21264245
Immunity. 2016 Aug 16;45(2):402-14
pubmed: 27473412
Immunity. 1998 Jul;9(1):81-91
pubmed: 9697838
Annu Rev Biochem. 2007;76:1-22
pubmed: 17328676
J Exp Med. 2016 May 2;213(5):733-50
pubmed: 27069113
Am J Transplant. 2013 Jun;13(6):1503-11
pubmed: 23570303
Nat Rev Immunol. 2007 Aug;7(8):633-43
pubmed: 17641666
Cell Immunol. 2017 Nov;321:8-17
pubmed: 28838763
Nat Commun. 2018 May 1;9(1):1758
pubmed: 29717110
J Exp Med. 2014 Sep 22;211(10):1977-91
pubmed: 25180065
J Immunol. 2017 Mar 1;198(5):1921-1927
pubmed: 28093524
Cell Rep. 2019 Apr 30;27(5):1446-1460.e4
pubmed: 31042472
J Immunol. 2013 May 15;190(10):4982-90
pubmed: 23589617
J Clin Invest. 2005 Nov;115(11):3205-16
pubmed: 16211091
J Immunol. 2010 Dec 15;185(12):7146-50
pubmed: 21078902
Immunol Rev. 2019 Mar;288(1):149-160
pubmed: 30874358
Nature. 1991 Apr 4;350(6317):423-6
pubmed: 1901381
Immunity. 2018 Oct 16;49(4):725-739.e6
pubmed: 30314758
J Leukoc Biol. 2018 Feb;103(2):269-274
pubmed: 29345369
J Exp Med. 2008 Aug 4;205(8):1797-805
pubmed: 18625747
J Clin Invest. 2017 Apr 3;127(4):1392-1404
pubmed: 28240602
Arthritis Rheum. 2010 Jan;62(1):64-74
pubmed: 20039397
Nat Immunol. 2018 Apr;19(4):407-419
pubmed: 29483597
Arthritis Rheum. 2009 Nov;60(11):3251-6
pubmed: 19877054
Eur J Immunol. 2019 Jan;49(1):170-178
pubmed: 30353919
J Immunol. 2015 Sep 1;195(5):1933-7
pubmed: 26297793
Annu Rev Immunol. 2018 Apr 26;36:339-357
pubmed: 29356584
Ann Rheum Dis. 2009 Jun;68(6):1011-6
pubmed: 18647852
Immunity. 2007 May;26(5):629-41
pubmed: 17509906
Clin Immunol. 2015 Jan;156(1):65-73
pubmed: 25463433
J Immunol. 2016 May 1;196(9):3525-31
pubmed: 27022196
J Exp Med. 2017 Nov 6;214(11):3207-3217
pubmed: 28899868
Science. 2008 Aug 1;321(5889):696-9
pubmed: 18566249
Nat Immunol. 2009 Dec;10(12):1292-9
pubmed: 19855380
J Exp Med. 2016 Jun 27;213(7):1255-65
pubmed: 27298445