Rapid adaptation of signaling networks in the fungal pathogen Magnaporthe oryzae.
Adaptation, Physiological
/ genetics
Dioxoles
/ pharmacology
Drug Resistance, Fungal
/ genetics
Fungal Proteins
/ genetics
Gene Expression Profiling
Gene Expression Regulation, Fungal
Gene Regulatory Networks
Genome, Fungal
/ genetics
Glycerol
/ metabolism
Loss of Function Mutation
Magnaporthe
/ drug effects
Oryza
/ microbiology
Osmoregulation
/ genetics
Plant Diseases
/ microbiology
Pyrroles
/ pharmacology
Salt Stress
Signal Transduction
/ genetics
Epigenetics
Evolution of signaling networks
HOG pathway
Magnaporthe oryzae
Rapid adaptation
Reestablishment of osmoregulation
Rewiring
Suppressor
Journal
BMC genomics
ISSN: 1471-2164
Titre abrégé: BMC Genomics
Pays: England
ID NLM: 100965258
Informations de publication
Date de publication:
22 Oct 2019
22 Oct 2019
Historique:
received:
19
03
2019
accepted:
20
09
2019
entrez:
24
10
2019
pubmed:
24
10
2019
medline:
27
2
2020
Statut:
epublish
Résumé
One fundamental question in biology is how the evolution of eukaryotic signaling networks has taken place. "Loss of function" (lof) mutants from components of the high osmolarity glycerol (HOG) signaling pathway in the filamentous fungus Magnaporthe oryzae are viable, but impaired in osmoregulation. After long-term cultivation upon high osmolarity, stable individuals with reestablished osmoregulation capacity arise independently from each of the mutants with inactivated HOG pathway. This phenomenon is extremely reproducible and occurs only in osmosensitive mutants related to the HOG pathway - not in other osmosensitive Magnaporthe mutants. The major compatible solute produced by these adapted strains to cope with high osmolarity is glycerol, whereas it is arabitol in the wildtype strain. Genome and transcriptome analysis resulted in candidate genes related to glycerol metabolism, perhaps responsible for an epigenetic induced reestablishment of osmoregulation, since these genes do not show structural variations within the coding or promotor sequences. This is the first report of a stable adaptation in eukaryotes by producing different metabolites and opens a door for the scientific community since the HOG pathway is worked on intensively in many eukaryotic model organisms.
Sections du résumé
BACKGROUND
BACKGROUND
One fundamental question in biology is how the evolution of eukaryotic signaling networks has taken place. "Loss of function" (lof) mutants from components of the high osmolarity glycerol (HOG) signaling pathway in the filamentous fungus Magnaporthe oryzae are viable, but impaired in osmoregulation.
RESULTS
RESULTS
After long-term cultivation upon high osmolarity, stable individuals with reestablished osmoregulation capacity arise independently from each of the mutants with inactivated HOG pathway. This phenomenon is extremely reproducible and occurs only in osmosensitive mutants related to the HOG pathway - not in other osmosensitive Magnaporthe mutants. The major compatible solute produced by these adapted strains to cope with high osmolarity is glycerol, whereas it is arabitol in the wildtype strain. Genome and transcriptome analysis resulted in candidate genes related to glycerol metabolism, perhaps responsible for an epigenetic induced reestablishment of osmoregulation, since these genes do not show structural variations within the coding or promotor sequences.
CONCLUSION
CONCLUSIONS
This is the first report of a stable adaptation in eukaryotes by producing different metabolites and opens a door for the scientific community since the HOG pathway is worked on intensively in many eukaryotic model organisms.
Identifiants
pubmed: 31640564
doi: 10.1186/s12864-019-6113-3
pii: 10.1186/s12864-019-6113-3
pmc: PMC6805500
doi:
Substances chimiques
Dioxoles
0
Fungal Proteins
0
Pyrroles
0
fludioxonil
ENS9J0YM16
Glycerol
PDC6A3C0OX
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
763Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : 403841309
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