Multimeric Purinoceptor Detection by Bioluminescence Resonance Energy Transfer.
BRET
BiFC
Bioluminescence
Heteromer
P2X
Stoichiometry
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
25
10
2019
pubmed:
28
10
2019
medline:
18
4
2020
Statut:
ppublish
Résumé
Assays based on bioluminescence resonance energy transfer (BRET) provide a sensitive and simple method to study protein-protein interactions in live cells. Here we describe a protocol using BRET technique to investigate potential interactions between P2X subunits. This approach combined with bimolecular fluorescence complementation (BiFC) can also be employed to determine the stoichiometry of heteromeric P2X receptors.
Identifiants
pubmed: 31646487
doi: 10.1007/978-1-4939-9717-6_11
doi:
Substances chimiques
Receptors, Purinergic P2X
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM