Application of Fluorescent Purinoceptor Antagonists for Bioluminescence Resonance Energy Transfer Assays and Fluorescent Microscopy.
Bioluminescence Resonance Energy Transfer Techniques
/ methods
Fluorescence
HEK293 Cells
Humans
Luciferases
/ metabolism
Microscopy, Fluorescence
/ methods
Protein Binding
Protein Multimerization
Purinergic P1 Receptor Agonists
/ chemistry
Receptor, Adenosine A3
/ chemistry
Receptors, Purinergic P1
/ chemistry
Signal Transduction
Antagonist
BRET
Confocal microscopy
Fluorescence
Ligand binding
Purinergic receptor
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
25
10
2019
pubmed:
28
10
2019
medline:
18
4
2020
Statut:
ppublish
Résumé
Fluorescent antagonists offer the ability to interrogate G protein-coupled receptor pharmacology. With resonance energy transfer techniques, fluorescent antagonists can be implemented to monitor receptor-ligand interactions using assays originally designed for radiolabeled probes. The fluorescent nature of these antagonists also enables the localization and distribution of the receptors to be visualized in living cells. Here, we describe the generation of modified purinergic receptors with the NanoLuc luciferase or SNAP-tag, using the P1 adenosine A
Identifiants
pubmed: 31646488
doi: 10.1007/978-1-4939-9717-6_12
doi:
Substances chimiques
Purinergic P1 Receptor Agonists
0
Receptor, Adenosine A3
0
Receptors, Purinergic P1
0
Luciferases
EC 1.13.12.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
163-181Subventions
Organisme : Medical Research Council
ID : MR/N020081/1
Pays : United Kingdom