Dendritic Cell Vaccination in Metastatic Melanoma Turns "Non-T Cell Inflamed" Into "T-Cell Inflamed" Tumors.
Adult
Aged
CD8-Positive T-Lymphocytes
/ immunology
Cancer Vaccines
/ administration & dosage
Dendritic Cells
/ immunology
Female
Follow-Up Studies
Humans
Inflammation
/ immunology
Lymphocytes, Tumor-Infiltrating
/ immunology
Male
Melanoma
/ immunology
Middle Aged
Neoplasm Metastasis
T-Lymphocytes, Regulatory
/ immunology
Vaccination
PDL1
T cell landscape
dendritic cell vaccine
immunomonitoring
immunotherapy
melanoma
tumor microenvironment
Journal
Frontiers in immunology
ISSN: 1664-3224
Titre abrégé: Front Immunol
Pays: Switzerland
ID NLM: 101560960
Informations de publication
Date de publication:
2019
2019
Historique:
received:
15
07
2019
accepted:
18
09
2019
entrez:
26
10
2019
pubmed:
28
10
2019
medline:
29
10
2020
Statut:
epublish
Résumé
Dendritic cell (DC)-based vaccination effectively induces anti-tumor immunity, although in the majority of cases this does not translate into a durable clinical response. However, DC vaccination is characterized by a robust safety profile, making this treatment a potential candidate for effective combination cancer immunotherapy. To explore this possibility, understanding changes occurring in the tumor microenvironment (TME) upon DC vaccination is required. In this line, quantitative and qualitative changes in tumor-infiltrating T lymphocytes (TILs) induced by vaccination with autologous tumor lysate/homogenate loaded DCs were investigated in a series of 16 patients with metastatic melanoma. Immunohistochemistry for CD4, CD8, Foxp3, Granzyme B (GZMB), PDL1, and HLA class I was performed in tumor biopsies collected before and after DC vaccination. The density of each marker was quantified by automated digital pathology analysis on whole slide images. Co-expression of markers defining functional phenotypes, i.e., Foxp3
Identifiants
pubmed: 31649669
doi: 10.3389/fimmu.2019.02353
pmc: PMC6794451
doi:
Substances chimiques
Cancer Vaccines
0
Banques de données
figshare
['10.6084/m9.figshare.c.4584041.v3']
Types de publication
Clinical Trial
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2353Informations de copyright
Copyright © 2019 Bulgarelli, Tazzari, Granato, Ridolfi, Maiocchi, de Rosa, Petrini, Pancisi, Gentili, Vergani, Piccinini, Carbonaro, Leone, Foschi, Ancarani, Framarini and Guidoboni.
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