Human amniotic fluid stem cells attract osteoprogenitor cells in bone healing.
Amniotic Fluid
/ cytology
Animals
Bone Regeneration
Cell Communication
Cell Lineage
Cell Movement
Cell Tracking
Cells, Cultured
Coculture Techniques
Female
Genes, Reporter
Humans
Male
Mice, Inbred NOD
Mice, SCID
Mice, Transgenic
Models, Animal
Osteogenesis
Phenotype
Pregnancy
Signal Transduction
Skull
/ metabolism
Stem Cell Transplantation
Stem Cells
/ metabolism
Time Factors
bone
mice
regenerative medicine
stem cell transplantation
transgenic
Journal
Journal of cellular physiology
ISSN: 1097-4652
Titre abrégé: J Cell Physiol
Pays: United States
ID NLM: 0050222
Informations de publication
Date de publication:
05 2020
05 2020
Historique:
received:
30
04
2019
accepted:
30
09
2019
pubmed:
28
10
2019
medline:
9
3
2021
entrez:
26
10
2019
Statut:
ppublish
Résumé
Current treatments of large bone defects are based on autologous or allogenic bone transplantation. Human amniotic fluid stem cells (hAFSCs) were evaluated for their potential in bone regenerative medicine. In this study, hAFSCs were transduced with lentiviral vector harboring red fluorescent protein to investigate their role in the regeneration of critical-size bone defects in calvarial mouse model. To distinguish donor versus recipient cells, a transgenic mouse model carrying GFP fluorescent reporter was used as recipient to follow the fate of hAFSCs transplanted in vivo into Healos® scaffold. Our results showed that transduced hAFSCs can be tracked in vivo directly at the site of transplantation. The presence of GFP positive cells in the scaffold at 3 and 6 weeks after transplantation indicates that donor hAFSCs can recruit host cells during the repair process. These observations help clarify the role of hAFSCs in bone tissue repair.
Identifiants
pubmed: 31650536
doi: 10.1002/jcp.29342
pmc: PMC7018542
mid: NIHMS1058908
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4643-4654Subventions
Organisme : NIAMS NIH HHS
ID : R01 AR055607
Pays : United States
Organisme : NIAMS NIH HHS
ID : R01 AR070813
Pays : United States
Informations de copyright
© 2019 Wiley Periodicals, Inc.
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