Assay Integrity of a PCR Influenza Point-of-Care Test Remains Following Artificial System Contamination.
Journal
The journal of applied laboratory medicine
ISSN: 2576-9456
Titre abrégé: J Appl Lab Med
Pays: England
ID NLM: 101693884
Informations de publication
Date de publication:
11 2019
11 2019
Historique:
received:
10
12
2018
accepted:
08
04
2019
entrez:
30
10
2019
pubmed:
30
10
2019
medline:
20
9
2020
Statut:
ppublish
Résumé
Healthcare providers who have access to tests at the point of care (POC) are increasingly requesting the same performance from the POC test as they expect from the laboratory. With the introduction of the cobas To assess the likelihood of system contamination contributing to the generation of false-positive results, in this study we contaminated a cobas Liat System with flu A/B-positive control material. The system contamination was then assessed by swabbing exposed surfaces. Following confirmed system contamination, negative control samples were processed to determine whether system contamination had an impact on the expected negative results. Instrument contamination was confirmed, and no detectable flu A/B signal was observed for any of the negative control tubes run immediately following confirmation of system contamination. Environmental contamination of the Liat instrument does not have an impact on the integrity of the result.
Sections du résumé
BACKGROUND
Healthcare providers who have access to tests at the point of care (POC) are increasingly requesting the same performance from the POC test as they expect from the laboratory. With the introduction of the cobas
METHODS
To assess the likelihood of system contamination contributing to the generation of false-positive results, in this study we contaminated a cobas Liat System with flu A/B-positive control material. The system contamination was then assessed by swabbing exposed surfaces. Following confirmed system contamination, negative control samples were processed to determine whether system contamination had an impact on the expected negative results.
RESULTS
Instrument contamination was confirmed, and no detectable flu A/B signal was observed for any of the negative control tubes run immediately following confirmation of system contamination.
CONCLUSION
Environmental contamination of the Liat instrument does not have an impact on the integrity of the result.
Identifiants
pubmed: 31659080
pii: jalm.2018.028639
doi: 10.1373/jalm.2018.028639
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
422-426Informations de copyright
© 2019 American Association for Clinical Chemistry.