Reduced Cholinergic Activity in the Hippocampus of Hippocampal Cholinergic Neurostimulating Peptide Precursor Protein Knockout Mice.
Acetylcholine
/ metabolism
Animals
Axons
/ metabolism
Behavior Rating Scale
CA1 Region, Hippocampal
/ cytology
Choline O-Acetyltransferase
/ metabolism
Cholinergic Neurons
/ physiology
Male
Mice
Mice, Inbred C57BL
Mice, Knockout
Neuropeptides
/ metabolism
Phosphatidylethanolamine Binding Protein
/ genetics
Raf kinase inhibitory protein (RKIP)
cholineacetyltransferase (ChAT)
hippocampal cholinergic neurostimulating peptide (HCNP)
hippocampal cholinergic neurostimulating peptide precursor protein (HCNP-pp)
hippocampal cholinergic neurostimulating peptide precursor protein knockout mouse (HCNP-pp KO mouse)
local field potential (LFP)
phosphatidylethanolamine-binding protein 1 (PEBP1)
theta oscillation
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
28 Oct 2019
28 Oct 2019
Historique:
received:
30
08
2019
revised:
24
10
2019
accepted:
25
10
2019
entrez:
31
10
2019
pubmed:
31
10
2019
medline:
31
3
2020
Statut:
epublish
Résumé
The cholinergic efferent network from the medial septal nucleus to the hippocampus has an important role in learning and memory processes. This cholinergic projection can generate theta oscillations in the hippocampus to efficiently encode novel information. Hippocampal cholinergic neurostimulating peptide (HCNP) induces acetylcholine synthesis in medial septal nuclei. HCNP is processed from the N-terminal region of a 186 amino acid, 21 kD HCNP precursor protein called HCNP-pp (also known as Raf kinase inhibitory protein (RKIP) and phosphatidylethanolamine-binding protein 1 (PEBP1)). In this study, we generated HCNP-pp knockout (KO) mice and assessed their cholinergic septo-hippocampal projection, local field potentials in CA1, and behavioral phenotypes. No significant behavioral phenotype was observed in HCNP-pp KO mice. However, theta power in the CA1 of HCNP-pp KO mice was significantly reduced because of fewer cholineacetyltransferase-positive axons in the CA1 stratum oriens. These observations indicated disruption of cholinergic activity in the septo-hippocampal network. Our study demonstrates that HCNP may be a cholinergic regulator in the septo-hippocampal network.
Identifiants
pubmed: 31661900
pii: ijms20215367
doi: 10.3390/ijms20215367
pmc: PMC6862429
pii:
doi:
Substances chimiques
Neuropeptides
0
Phosphatidylethanolamine Binding Protein
0
hippocampal cholinergic neurostimulating peptide
140158-49-2
Choline O-Acetyltransferase
EC 2.3.1.6
Acetylcholine
N9YNS0M02X
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Japan Society for the Promotion of Science
ID : 17K09761
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
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