TSPAN5 Enriched Microdomains Provide a Platform for Dendritic Spine Maturation through Neuroligin-1 Clustering.


Journal

Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691

Informations de publication

Date de publication:
29 10 2019
Historique:
received: 16 04 2018
revised: 09 07 2019
accepted: 18 09 2019
entrez: 31 10 2019
pubmed: 31 10 2019
medline: 26 9 2020
Statut: ppublish

Résumé

Tetraspanins are a class of evolutionarily conserved transmembrane proteins with 33 members identified in mammals that have the ability to organize specific membrane domains, named tetraspanin-enriched microdomains (TEMs). Despite the relative abundance of different tetraspanins in the CNS, few studies have explored their role at synapses. Here, we investigate the function of TSPAN5, a member of the tetraspanin superfamily for which mRNA transcripts are found at high levels in the mouse brain. We demonstrate that TSPAN5 is localized in dendritic spines of pyramidal excitatory neurons and that TSPAN5 knockdown induces a dramatic decrease in spine number because of defects in the spine maturation process. Moreover, we show that TSPAN5 interacts with the postsynaptic adhesion molecule neuroligin-1, promoting its correct surface clustering. We propose that membrane compartmentalization by tetraspanins represents an additional mechanism for regulating excitatory synapses.

Identifiants

pubmed: 31665629
pii: S2211-1247(19)31239-2
doi: 10.1016/j.celrep.2019.09.051
pmc: PMC6899445
pii:
doi:

Substances chimiques

Cell Adhesion Molecules, Neuronal 0
TSPAN5 protein, human 0
Tetraspanins 0
neuroligin 1 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1130-1146.e8

Subventions

Organisme : Wellcome Trust
Pays : United Kingdom
Organisme : UK Medical Research Council
Pays : International

Informations de copyright

Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.

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Auteurs

Edoardo Moretto (E)

CNR, Institute of Neuroscience, Milan 20129, Italy; BioMETRA, Department of Medical Biotechnology and Translational Medicine, University of Milan, Milan, Italy; UK Dementia Research Institute at University College London, London WC1E 6BT, UK.

Anna Longatti (A)

CNR, Institute of Neuroscience, Milan 20129, Italy; DiSFeB, Department of Pharmacological and Biomolecular Sciences, University of Milan, Milan, Italy.

Luca Murru (L)

CNR, Institute of Neuroscience, Milan 20129, Italy.

Ingrid Chamma (I)

Interdisciplinary Institute for Neuroscience, UMR 5297, Centre National de la Recherche Scientifique, Bordeaux, France; Interdisciplinary Institute for Neuroscience, University of Bordeaux, Bordeaux, France.

Alessandro Sessa (A)

Stem Cell and Neurogenesis Unit, Division of Neuroscience, San Raffaele Scientific Institute, 20132 Milan, Italy.

Jonathan Zapata (J)

CNR, Institute of Neuroscience, Milan 20129, Italy.

Eric Hosy (E)

Interdisciplinary Institute for Neuroscience, UMR 5297, Centre National de la Recherche Scientifique, Bordeaux, France; Interdisciplinary Institute for Neuroscience, University of Bordeaux, Bordeaux, France.

Matthieu Sainlos (M)

Interdisciplinary Institute for Neuroscience, UMR 5297, Centre National de la Recherche Scientifique, Bordeaux, France; Interdisciplinary Institute for Neuroscience, University of Bordeaux, Bordeaux, France.

Julien Saint-Pol (J)

INSERM, U935, 94807 Villejuif, France.

Eric Rubinstein (E)

INSERM, U935, 94807 Villejuif, France.

Daniel Choquet (D)

Interdisciplinary Institute for Neuroscience, UMR 5297, Centre National de la Recherche Scientifique, Bordeaux, France; Interdisciplinary Institute for Neuroscience, University of Bordeaux, Bordeaux, France; Bordeaux Imaging Center, UMS3420, CNRS, University of Bordeaux, US4 INSERM, Bordeaux, France.

Vania Broccoli (V)

Stem Cell and Neurogenesis Unit, Division of Neuroscience, San Raffaele Scientific Institute, 20132 Milan, Italy.

Giampietro Schiavo (G)

UK Dementia Research Institute at University College London, London WC1E 6BT, UK; Department of Neuromuscular Diseases, UCL Queen Square Institute of Neurology, University College London, London WC1N 3BG, UK; Discoveries Centre for Regenerative and Precision Medicine, University College London Campus, London WC1N 3BG, UK.

Olivier Thoumine (O)

Interdisciplinary Institute for Neuroscience, UMR 5297, Centre National de la Recherche Scientifique, Bordeaux, France; Interdisciplinary Institute for Neuroscience, University of Bordeaux, Bordeaux, France.

Maria Passafaro (M)

CNR, Institute of Neuroscience, Milan 20129, Italy. Electronic address: maria.passafaro@in.cnr.it.

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Classifications MeSH