Attacking Latent HIV with convertibleCAR-T Cells, a Highly Adaptable Killing Platform.


Journal

Cell
ISSN: 1097-4172
Titre abrégé: Cell
Pays: United States
ID NLM: 0413066

Informations de publication

Date de publication:
31 10 2019
Historique:
received: 16 05 2019
revised: 19 07 2019
accepted: 01 10 2019
entrez: 1 11 2019
pubmed: 2 11 2019
medline: 28 5 2020
Statut: ppublish

Résumé

Current approaches to reducing the latent HIV reservoir entail first reactivating virus-containing cells to become visible to the immune system. A critical second step is killing these cells to reduce reservoir size. Endogenous cytotoxic T-lymphocytes (CTLs) may not be adequate because of cellular exhaustion and the evolution of CTL-resistant viruses. We have designed a universal CAR-T cell platform based on CTLs engineered to bind a variety of broadly neutralizing anti-HIV antibodies. We show that this platform, convertibleCAR-T cells, effectively kills HIV-infected, but not uninfected, CD4 T cells from blood, tonsil, or spleen and only when armed with anti-HIV antibodies. convertibleCAR-T cells also kill within 48 h more than half of the inducible reservoir found in blood of HIV-infected individuals on antiretroviral therapy. The modularity of convertibleCAR-T cell system, which allows multiplexing with several anti-HIV antibodies yielding greater breadth and control, makes it a promising tool for attacking the latent HIV reservoir.

Identifiants

pubmed: 31668804
pii: S0092-8674(19)31118-3
doi: 10.1016/j.cell.2019.10.002
pmc: PMC6922308
mid: NIHMS1544021
pii:
doi:

Substances chimiques

Antibodies, Anti-Idiotypic 0

Types de publication

Journal Article Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

880-894.e10

Subventions

Organisme : NIDCR NIH HHS
ID : DP1 DE024408
Pays : United States
Organisme : NIAID NIH HHS
ID : P30 AI027763
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI109593
Pays : United States

Informations de copyright

Copyright © 2019 Elsevier Inc. All rights reserved.

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Auteurs

Eytan Herzig (E)

Gladstone Center for HIV Cure Research, Gladstone Institute of Virology and Immunology, San Francisco, CA 94158, USA; Departments of Medicine and Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA.

Kaman Chan Kim (KC)

Xyphos Biosciences, Inc., South San Francisco, CA 94080, USA.

Thomas A Packard (TA)

Gladstone Center for HIV Cure Research, Gladstone Institute of Virology and Immunology, San Francisco, CA 94158, USA; Departments of Medicine and Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA.

Noam Vardi (N)

Gladstone Center for Cell Circuitry, Gladstone Institutes, San Francisco, CA 94158, USA; Departments of Biochemistry and Biophysics and Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94158, USA.

Roland Schwarzer (R)

Gladstone Center for HIV Cure Research, Gladstone Institute of Virology and Immunology, San Francisco, CA 94158, USA; Departments of Medicine and Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA.

Andrea Gramatica (A)

Gladstone Center for HIV Cure Research, Gladstone Institute of Virology and Immunology, San Francisco, CA 94158, USA; Departments of Medicine and Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA.

Steven G Deeks (SG)

Department of Medicine, University of California, San Francisco, San Francisco, CA 94110, USA.

Steven R Williams (SR)

Xyphos Biosciences, Inc., South San Francisco, CA 94080, USA.

Kyle Landgraf (K)

Xyphos Biosciences, Inc., South San Francisco, CA 94080, USA.

Nigel Killeen (N)

Xyphos Biosciences, Inc., South San Francisco, CA 94080, USA.

David W Martin (DW)

Xyphos Biosciences, Inc., South San Francisco, CA 94080, USA.

Leor S Weinberger (LS)

Gladstone Center for HIV Cure Research, Gladstone Institute of Virology and Immunology, San Francisco, CA 94158, USA; Gladstone Center for Cell Circuitry, Gladstone Institutes, San Francisco, CA 94158, USA; Departments of Biochemistry and Biophysics and Pharmaceutical Chemistry, University of California, San Francisco, San Francisco, CA 94158, USA.

Warner C Greene (WC)

Gladstone Center for HIV Cure Research, Gladstone Institute of Virology and Immunology, San Francisco, CA 94158, USA; Departments of Medicine and Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA. Electronic address: warner.greene@gladstone.ucsf.edu.

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