New approach to measuring oxygen diffusion and consumption in encapsulated living cells, based on electron spin resonance microscopy.
Cell microencapsulation
Electron spin resonance imaging
Extracellular matrix
Oxygen diffusion
Journal
Acta biomaterialia
ISSN: 1878-7568
Titre abrégé: Acta Biomater
Pays: England
ID NLM: 101233144
Informations de publication
Date de publication:
01 01 2020
01 01 2020
Historique:
received:
16
07
2019
revised:
06
10
2019
accepted:
23
10
2019
pubmed:
2
11
2019
medline:
6
1
2021
entrez:
2
11
2019
Statut:
ppublish
Résumé
Cell microencapsulation within biocompatible polymers is an established technology for immobilizing living cells that secrete therapeutic products. These can be transplanted into a desired site in the body for the controlled and continuous delivery of the therapeutic molecules. One of the most important properties of the material that makes up the microcapsule is its oxygen penetrability, which is critical for the cells' survival. Oxygen reaches the cells inside the microcapsules via a diffusion process. The diffusion coefficient for the microcapsules' gel material is commonly measured using bulk techniques, where the gel in a chamber is first flushed with nitrogen and the subsequent rate of oxygen diffusion back into it is measured by an oxygen electrode placed in the chamber. This technique does not address possible heterogeneities between microcapsules, and also cannot reveal O
Identifiants
pubmed: 31672586
pii: S1742-7061(19)30711-1
doi: 10.1016/j.actbio.2019.10.032
pii:
doi:
Substances chimiques
Capsules
0
Oxygen
S88TT14065
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
384-394Informations de copyright
Copyright © 2019. Published by Elsevier Ltd.