Clathrin light chain A drives selective myosin VI recruitment to clathrin-coated pits under membrane tension.
Actins
/ metabolism
Adaptor Proteins, Signal Transducing
/ metabolism
Caco-2 Cells
Cell Culture Techniques
Clathrin Light Chains
/ metabolism
Clathrin-Coated Vesicles
/ metabolism
Coated Pits, Cell-Membrane
/ metabolism
Cysts
Endocytosis
Humans
Magnetic Resonance Spectroscopy
Microfilament Proteins
/ metabolism
Myosin Heavy Chains
/ metabolism
Protein Binding
Protein Conformation
Protein Isoforms
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
31 10 2019
31 10 2019
Historique:
received:
03
06
2019
accepted:
27
09
2019
entrez:
2
11
2019
pubmed:
2
11
2019
medline:
4
3
2020
Statut:
epublish
Résumé
Clathrin light chains (CLCa and CLCb) are major constituents of clathrin-coated vesicles. Unique functions for these evolutionary conserved paralogs remain elusive, and their role in clathrin-mediated endocytosis in mammalian cells is debated. Here, we find and structurally characterize a direct and selective interaction between CLCa and the long isoform of the actin motor protein myosin VI, which is expressed exclusively in highly polarized tissues. Using genetically-reconstituted Caco-2 cysts as proxy for polarized epithelia, we provide evidence for coordinated action of myosin VI and CLCa at the apical surface where these proteins are essential for fission of clathrin-coated pits. We further find that myosin VI and Huntingtin-interacting protein 1-related protein (Hip1R) are mutually exclusive interactors with CLCa, and suggest a model for the sequential function of myosin VI and Hip1R in actin-mediated clathrin-coated vesicle budding.
Identifiants
pubmed: 31672988
doi: 10.1038/s41467-019-12855-6
pii: 10.1038/s41467-019-12855-6
pmc: PMC6823378
doi:
Substances chimiques
Actins
0
Adaptor Proteins, Signal Transducing
0
Clathrin Light Chains
0
HIP1R protein, human
0
Microfilament Proteins
0
Protein Isoforms
0
myosin VI
0
Myosin Heavy Chains
EC 3.6.4.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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