An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms.

Animals Clustered Regularly Interspaced Short Palindromic Repeats DNA / genetics DNA, Single-Stranded / genetics Drosophila Gene Knock-In Techniques / methods Homologous Recombination Mutagenesis, Insertional / methods
CRIMIC CRISPR D. melanogaster drop-in drosophila genetics genomics homologous recombination

Journal

eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614

Informations de publication

Date de publication:
01 11 2019
Historique:
received: 09 09 2019
accepted: 31 10 2019
pubmed: 2 11 2019
medline: 9 4 2020
entrez: 2 11 2019
Statut: epublish

Résumé

We previously reported a CRISPR-mediated knock-in strategy into introns of

Identifiants

DOI: 10.7554/eLife.51539 PMID: 31674908 PMC: PMC6855806
pubmed: 31674908
doi: 10.7554/eLife.51539
pii: 51539
pmc: PMC6855806
doi:
pii:

Substances chimiques

DNA, Single-Stranded 0
DNA 9007-49-2

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NIGMS NIH HHS
ID : P41 GM132087
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01GM067858
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM067858
Pays : United States
Organisme : NIH Office of the Director
ID : R01GM067858
Pays : International
Organisme : NIGMS NIH HHS
ID : R01 GM084947
Pays : United States
Organisme : NIH HHS
ID : R24 OD019847
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM067761
Pays : United States

Informations de copyright

© 2019, Kanca et al.

Déclaration de conflit d'intérêts

OK, JZ, JG, SK, DY, GA, HC, ZZ, LM, YH, WL, YF, MG, SY, KS, YH, AS, SM, NP No competing interests declared, HB Reviewing editor, eLife

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Auteurs

Oguz Kanca (O)

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, United States.
Jan and Dan Duncan Neurological Research Institute, Texas Children's Hospital, Houston, United States.
ORCID: 0000-0001-5438-0879

Jonathan Zirin (J)

Howard Hughes Medical Institute, Harvard Medical School, Boston, United States.
Department of Genetics, Harvard Medical School, Boston, United States.

Jorge Garcia-Marques (J)

Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, United States.

Shannon Marie Knight (SM)

Howard Hughes Medical Institute, Harvard Medical School, Boston, United States.
Department of Genetics, Harvard Medical School, Boston, United States.

Donghui Yang-Zhou (D)

Howard Hughes Medical Institute, Harvard Medical School, Boston, United States.
Department of Genetics, Harvard Medical School, Boston, United States.

Gabriel Amador (G)

Howard Hughes Medical Institute, Harvard Medical School, Boston, United States.
Department of Genetics, Harvard Medical School, Boston, United States.

Hyunglok Chung (H)

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, United States.
Jan and Dan Duncan Neurological Research Institute, Texas Children's Hospital, Houston, United States.

Zhongyuan Zuo (Z)

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, United States.
Jan and Dan Duncan Neurological Research Institute, Texas Children's Hospital, Houston, United States.

Liwen Ma (L)

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, United States.

Yuchun He (Y)

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, United States.
Howard Hughes Medical Institute, Baylor College of Medicine, Houston, United States.

Wen-Wen Lin (WW)

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, United States.

Ying Fang (Y)

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, United States.

Ming Ge (M)

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, United States.

Shinya Yamamoto (S)

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, United States.
Jan and Dan Duncan Neurological Research Institute, Texas Children's Hospital, Houston, United States.
Program in Developmental Biology, Baylor College of Medicine, Houston, United States.
Department of Neuroscience, Baylor College of Medicine, Houston, United States.

Karen L Schulze (KL)

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, United States.
Jan and Dan Duncan Neurological Research Institute, Texas Children's Hospital, Houston, United States.
Howard Hughes Medical Institute, Baylor College of Medicine, Houston, United States.
ORCID: 0000-0002-1368-729X

Yanhui Hu (Y)

Howard Hughes Medical Institute, Harvard Medical School, Boston, United States.
Department of Genetics, Harvard Medical School, Boston, United States.

Allan C Spradling (AC)

Department of Embryology, Howard Hughes Medical Institute, Carnegie Institution for Science, Baltimore, United States.
ORCID: 0000-0002-5251-1801

Stephanie E Mohr (SE)

Howard Hughes Medical Institute, Harvard Medical School, Boston, United States.
Department of Genetics, Harvard Medical School, Boston, United States.
ORCID: 0000-0001-9639-7708

Norbert Perrimon (N)

Howard Hughes Medical Institute, Harvard Medical School, Boston, United States.
Department of Genetics, Harvard Medical School, Boston, United States.
ORCID: 0000-0001-7542-472X

Hugo J Bellen (HJ)

Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, United States.
Jan and Dan Duncan Neurological Research Institute, Texas Children's Hospital, Houston, United States.
Howard Hughes Medical Institute, Baylor College of Medicine, Houston, United States.
Program in Developmental Biology, Baylor College of Medicine, Houston, United States.
Department of Neuroscience, Baylor College of Medicine, Houston, United States.
ORCID: 0000-0001-5992-5989

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › An efficient CRISPR-based strategy to insert...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Séquences répétées en tandem › Clustered regularly interspaced short palindromic repeats › An efficient CRISPR-based strategy to insert...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › An efficient CRISPR-based strategy to insert...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Conformation d'acide nucléique › ADN simple brin › An efficient CRISPR-based strategy to insert...
questionsmedicales.fr › Eucaryotes › Animaux › Invertébrés › Arthropodes › Insectes › Pterygota › Neoptera › Holometabola › Diptera › Drosophilidae › Drosophila › An efficient CRISPR-based strategy to insert...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Ciblage de gène › Techniques de knock-in de gènes › An efficient CRISPR-based strategy to insert...
questionsmedicales.fr › Phénomènes génétiques › Recombinaison génétique › Recombinaison homologue › An efficient CRISPR-based strategy to insert...
questionsmedicales.fr › Phénomènes génétiques › Mutagenèse › Mutagenèse par insertion › An efficient CRISPR-based strategy to insert...