Non-transmissible MV Vector with Segmented RNA Genome Establishes Different Types of iPSCs from Hematopoietic Cells.
Animals
Blood Donors
Cell Differentiation
/ genetics
Cellular Reprogramming
/ genetics
Fibroblasts
/ metabolism
Genetic Therapy
/ methods
Genetic Vectors
Genome, Viral
/ genetics
HEK293 Cells
Hematopoietic Stem Cells
/ metabolism
Heterografts
Humans
Induced Pluripotent Stem Cells
/ metabolism
Male
Measles virus
/ genetics
Mice
Mice, Inbred NOD
RNA, Viral
/ genetics
Sendai virus
/ genetics
T-Lymphocytes
/ metabolism
Transduction, Genetic
Transgenes
RNA virus
hematopoietic stem cell
iPSC
measles virus
naïve
non-integrating
non-transmissible
segmented RNA genome
vector
viral gene transfer vector
Journal
Molecular therapy : the journal of the American Society of Gene Therapy
ISSN: 1525-0024
Titre abrégé: Mol Ther
Pays: United States
ID NLM: 100890581
Informations de publication
Date de publication:
08 01 2020
08 01 2020
Historique:
received:
24
06
2018
revised:
30
08
2019
accepted:
06
09
2019
pubmed:
5
11
2019
medline:
22
12
2020
entrez:
4
11
2019
Statut:
ppublish
Résumé
Recent advances in gene therapy technologies have enabled the treatment of congenital disorders and cancers and facilitated the development of innovative methods, including induced pluripotent stem cell (iPSC) production and genome editing. We recently developed a novel non-transmissible and non-integrating measles virus (MV) vector capable of transferring multiple genes simultaneously into a wide range of cells through the CD46 and CD150 receptors. The MV vector expresses four genes for iPSC generation and the GFP gene for a period of time sufficient to establish iPSCs from human fibroblasts as well as peripheral blood T cells. The transgenes were expressed differentially depending on their gene order in the vector. Human hematopoietic stem/progenitor cells were directly and efficiently reprogrammed to naive-like cells that could proliferate and differentiate into primed iPSCs by the same method used to establish primed iPSCs from other cell types. The novel MV vector has several advantages for establishing iPSCs and potential future applications in gene therapy.
Identifiants
pubmed: 31677955
pii: S1525-0016(19)30410-1
doi: 10.1016/j.ymthe.2019.09.007
pmc: PMC6952176
pii:
doi:
Substances chimiques
RNA, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
129-141Informations de copyright
Copyright © 2019 The American Society of Gene and Cell Therapy. Published by Elsevier Inc. All rights reserved.
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